Virtual Microscope Questions

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Pueblo Community College *

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204

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Mechanical Engineering

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Dec 6, 2023

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Virtual Microscope Lab Questions Lisa Manco (Renz) S02979756 Define the following terms: Stage: main, flat surface that holds the slides for observation. Objective Lens: lens nearest to the object being viewed. Arm: area of the microscope between the tube and the base. Eye Piece: lens you look through, to present the image of the specimen. Base: bottom support structure of the microscope. Short Answer: 1. What role does immersion oil play in the use of a light Microscope? Serves as a bridge between the lenses and the glass plate containing the specimen helping to focus the light and increase the resolution. 2. How would you move the slide under the 10x objective lens? You would use the mechanical stage that allows the slide to be moved back and forth and side to side. 3. When positioning the slide under the lens, what steps do you follow? Place the stage as far away from the objective lenses as possible. Rotate the revolving nosepiece until the lowest power objective lens clicks into place. Looking at your stage from the side, place the specimen over the hole in the stage. Still looking from the side, lower the lowest objective lens (4X) using the coarse adjustment knob until it is as close as possible to the stage. Look through the eyepiece and slowly raise the objective lens using the coarse adjustment knob until your object comes into focus. To focus your
object at the next highest power look from the side of the stage and slowly rotate the objective lens (10X) into place and use the fine adjustment knob to bring your object into focus. To focus at the highest power, rotate the nosepiece to the objective lens (40X) while looking at it from the side. If you do not look at it from the side you may crack your slide or the objective lens. 4. What role do color bands play in objective lens? Visual indicator of the power of each objective. 4x is red, 10x is yellow, 40x is blue, 100x is white. 5. If you were interested in looking at an object at 200x total magnification, how would you go about this? You would use a 10x ocular lens and a 20x objective lens to get a 200x total magnification. 6. Describe how the letter ‘e’ would look under a scanning objective. It appears upside down and backwards.
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