Conduction Velocity_Report Sheet

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Apr 3, 2024

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HON 240: Conduction Velocity Follow the instructions in the report sheet, then save this file as a pdf . Upload it to Canvas. Ensure any data you show is clear, with an appropriate zoom. Use screen captures to transfer your data from the computer to this file. Do not include photos of computer screens – this makes it too difficult to see the data clearly. DUE February 28 by 11:59 pm The lab activity and much of the information herein are based on the Backyard Brains Website (see syllabus) and Shannon, Kyle M., Gregory J. Gage, Aleksandra Jankovic, W. Jeffrey Wilson, and Timothy C. Marzullo. “Portable Conduction Velocity Experiments Using Earthworms for the College and High School Neuroscience Teaching Laboratory.” Advances in Physiology Education 38, no. 1 (March 2014): 62–70.

HON 240: Conduction Velocity Experimental Setup – 10 points Paste a picture below of the experimental setup you used to acquire your data. Ensure that the photo clearly shows the placement of the electrodes in the worm. Stage the picture such that you are pretending to (or actually are) tap the worm. Ensure that this picture represents the actual experimental setup you used to acquire the data you will show in subsequent slides. Take your own photo; do not use someone else’s. You may ask someone to take your picture for you while you tap the worm.

HON 240: Conduction Velocity Spike Data A – 10 points Show data below where you tapped the front of the worm to elicit spikes in channels 1 and 2. Ensure that the zoom of the data is proper for purposes of determining conduction velocity. In the data shown, a spike should be clear in both channels 1 and 2, with the spike for channel 2 occurring before channel 1. The spike peaks should be highlighted such that the time difference between the spikes is displayed on the data. Note that you will repeat this for 2 more spikes – 3 spikes total. You need 3 different sets of spikes, you can’t re-use the same set of spike data twice.

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HON 240: Conduction Velocity Spike Data B – 10 points Show your second set of spike data below, with the same parameters as explained for Spike Data - A.

HON 240: Conduction Velocity Spike Data C – 10 points Show your second set of spike data below, with the same parameters as explained for Spike Data - A.

HON 240: Conduction Velocity Calculations – 30 points Record the distance between the electrodes in the experimental setup you used below. Next, enter the time lapsed between observing the spike at electrode 2 and electrode 1 for each of the three trials you showed in the previous slides (Spike Data, A, B, and C). Determine the conduction velocity for each set of Spike Data in m/s, then determine the average conduction velocity. You must show units to receive full credit. Distance between electrodes: Spike Data A time difference between spikes: Spike Data B time difference between spikes: Spike Data C time difference between spikes: Spike Data A conduction velocity: Spike Data B conduction velocity: Spike Data C conduction velocity: Average Conduction Velocity: Determine the precision of your conduction velocity measurements by determining the standard deviation. This is a measurement of how far apart your measurement are from each other. If the standard deviation is small, then your measurements are relatively close to each other and your measurement precision is higher. You can calculate standard deviation using most calculators or Excel. You can also use an online calculator such as this one: https://www.calculator.net/standard-deviation-calculator.html , where you can enter in the numbers for your spike A, B, and C conduction velocities, separated by a comma, and then tell it to calculate. Below, enter your final value for conduction velocity, which you will express as Average ± standard deviation. For example, if my average conduction velocity is 5.2 m/s and my standard deviation is 1, I would express my measurement as “ 5 ± 1 m/s) below. Conduction Velocity (Avg ± S.D.):

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HON 240: Conduction Velocity Questions – 10 points each For each of the questions below, record your answer below the question. Note that you must cite information sources used to answer each question. 1) Determining the conduction velocity of neurons in crickets would be much more difficult. However, suppose that we did successfully determine the conduction velocity of the cricket neuron(s) we observed in the previous lab. Would you expect the conduction velocity for these cricket neurons, on average, to be faster, slower, or roughly the same? Explain your answer. 2) The reason that our recorded action potentials do not look like the ideal action potential shown in the PowerPoint for the class is that our measurements are extracellular. Research this statement and briefly explain what this means below, in your own words. 3) We observed action potentials for the median giant fiber (MGF) of the worm. This is a neuron with a relatively large axon. Do some simple research and try to find an animal with very large neurons. Additionally, try to find an animal with many more neurons than our worms. Your source(s) for this question may be a non-scientific website. You still need to cite your sources though.