Experimental and results

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Michigan State University *

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161

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Chemistry

Date

Jan 9, 2024

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docx

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3

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Experimental Experimental The group made solutions at different concentrations. The concentrations used were 20%,40%,60%,80%. They were made by combining an amount of 100% concentrated dye with an amount of water in order to result in 10mL of a mixture solution. For 20% concentration, 2mL of dye was mixed with 8mL of water. For 40%, 4mL of dye was mixed with 6mL water. For 60%, 6mL of dye was mixed with 4mL of water. For 80%, 8mL of dye was mixed with 2mL of water. This entire process was done for all 7 dyes. A spectrophotometer was used in this experiment. First it was primed with water, then each concentration of each dye was individually loaded into the machine. Doing this gave us the lambda max and the absorption for each dye and each concentration. The replication was made by taking percent concentrations of the 2 dyes and multiplying it by 100mL to dilute the solution. [1]
Experimental Results Red Dye 40: The data collected for Red dye 40 can be found in the table in Figure 2. This includes the molar concentration and average absorbance at each dye solution concentration. The data is reflected in the graph in Figure 1. Figure 1 Figure 2 Blue Dye 1: The data collected for Blue dye 1 can be found in the table in Figure 4. This includes the molar concentration and average absorbance at each dye solution concentration. The data is reflected in the graph in Figure 3.
Experimental Figure 3 Figure 4 References: [1] Cooper, M.M.; Day, E.L.; Duffy, E.M.; Pollock, A.M.; Posey, L.A.; Ward, J.S. Cooperative Chemistry Student Laboratory Manual. Michigan State University, East Lansing, MI. Student laboratory manual, 2019
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