Unknown Project F23 (1)

Bacterial Unknown Project Part 1: Materials and Methods (5 points) - (SAVE 1 PAGE in notebook) * all scientific writing is written in 3rd person tense. Do not use “I” or “we”. In your lab notebook, on a new page of your notebook after the initial unknown results entry (Gram stains, streak plates), title the following and answer below each heading: Materials and Methods : Write one sentence per test performed that includes the media and reagents used. For example: “A Gram stain was performed using Gram’s Crystal Violet, Iodine, Acetone-alcohol, and Gram’s Safranin.” Use the course schedule as a checklist. Part 2: Results and Identification (25 points) (SAVE 3 PAGES in notebook) Identification of Unknown Bacteria : ( on a new page of your notebook ). A. Cut and paste the dichotomous keys associated with your bacteria. Highlight the flow of the dichotomous key to show how your bacteria unknowns were narrowed down to a related group or genus. You do not need to paste the results tables or the DIFCO identification chart. B. Use the table below for important test results used to identify your unknowns to species. You may cut and paste a digital version or use a hand drawn one. ONLY include relevant tests that YOU needed to identify each species. Record your relevant result and what it meant to your identification. For example, write: “The negative urease test result eliminated the Proteus, and Morganella genera.” in the third column. Use two tests to eliminate closely related species, if possible. You will need to add additional rows , depending on the number of tests that were needed in the identification of each species. The tests shown below are EXAMPLES, please modify as necessary for your unknowns. Name of Test Result (Pos/Neg) or other (describe) Significance of this result Gram-positive Unknown Catalase Positive The positive result eliminated the Enterococcus genus. Gram-negative Unknown Oxygen Requirement Facultative Anaerobe This result eliminated the Pseudomonas and Acinetobacter genera.

C. Write the Identity of your Bacterial Unknowns after the results table in your notebook. Gram Positive Unknown: Gram Negative Unknown: Part 3: Research on Unknown Bacteria (25 points) (SAVE 3 PAGES in notebook) Research on Unknown Bacteria : ( on a new page of your notebook). This must be your own work, not a collaborative effort done with your lab partner! A. Research your bacteria by using the Tortora textbook and the cdc.gov website . Use the table below to summarize your findings in your notebook. If you are using a closely related pathogen for diseases and virulence factors, write the genus and species in your notebook, above the table. You may cut and paste a digital version of the table or use a hand drawn one in your notebook. Modify the table as necessary. Virulence factors associated with your unknowns (or closely related organism). List and define the virulence factors in 1 sentence or descriptive phrase. Diseases associated with your unknowns (or closely related organism). Select 3 diseases caused by your bacteria. Briefly describe each disease in 1 sentence or descriptive phrase. Related Gram-positive Pathogen: Related Gram-negative Pathogen: Gram-positive Unknown -Virulence factor(s): Disease 1 Brief description Disease 2 Brief description Disease 3 Brief description Gram-positive Unknown -Virulence factor(s): Disease 1 Brief description Disease 2 Brief description Disease 3 Brief description Part 4: Journal articles: (25 points) (SAVE 2 PAGES in notebook) Journal Articles on Unknown Bacteria : (on a new page of your notebook). This must be your own work, not a collaborative effort done with your lab partner . 1) Find one recent (<10 yrs old) scientific study on each of your unknown bacteria (or closely related pathogen). 2) Cut and paste 1 results figure from each article into your lab notebook. Summarize the figure below it in your own words, in complete sentences, as if describing the figure to a classmate. Your description must