Mod1_ Enzymatic Activity Data Sheet sama abdelaziz

Data Sheet: Name: sama abdelaziz PS ID: 2161063 In this experiment, we will be comparing replicates of the same enzymatic reaction. State your hypothesis: The enzyme will cause the development of a yellow tint, and as the amount of time passes, more purple light will be absorbed. State the null hypothesis: There won't be a change when the enzyme is added, but purple light will be absorbed. 1. Table1 . Absorbance data for the production of ONP. Time O.D. 420 Tube 1 O.D. 420 Tube 2 O.D. 420 Tube 3 Mean O.D. 420 1 min 0.200 0.254 0.120 .1913 2 min 0.251 0.321 0.149 .2403 3 min 0.273 0.371 0.185 .2763 4 min 0.417 0.425 0.229 .3570 5 min 0.458 0.475 0.261 .3980 6 min 0.507 0.524 0.297 .4427 7 min 0.563 0.574 0.329 .4887 8 min 0.605 0.623 0.359 .5290 9 min 0.654 0.658 0.389 .5670 10 min 0.699 0.693 0.419 .6037 2. Use the grid below to plot your data. Label the axes appropriately. If you are drawing your graph using excel remove this grid and insert it here. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 1 2 3 4 5 6 7 8 9 10 Absorbance Minutes Beta-Galactosidase Mean Reaction Reaction is highest Reaction is lowest 3. Label the part of the curve where the rate of the reaction is highest. 4. Label the part of the curve where the rate of the reaction is lowest. 5. The highest rate is (estimate value, include units) .6037 OD 6. The lowest rate is (estimate value, include units) .1913 OD 7. Re-analyze your data—calculate the rate of the reaction for each 1 minute interval. A.I. Medrano, Ph.D. & D.L. Pattison, Ph.D. UH Department of Biology and Biochemistry. BIOL 1106 Lab

 Rate( x ) = OD/minute  Rate mean( x ): average of the rate for each time point for the 3 replicates  SD – standard deviation, s = √ ∑ ( x − x ) 2 n − 1 , where n = 3 Table 2 . Reaction Rate for the production of ONP Time Rate Tube 1 ( x 1 ) Rate Tube 2 ( x 2 ) Rate Tube 3 ( x 3 ) Rate Mean ( x ) S.D. ( s ) 1 min .2000 .2540 .1200 .1913 0.0674 2 min .1255 .1605 .0745 .1202 0.0432 3 min .0910 .1237 .0617 .0921 0.0310 4 min .1043 .1063 .0573 .0893 0.0277 5 min .0916 .0950 .0522 .0796 0.0238 6 min .0845 .0873 .0495 .0738 0.0211 7 min .0804 .0820 .0470 .0698 0.0198 8 min .0756 .0779 .0449 .0661 0.0184 9 min .0727 .0731 .0432 .0630 0.0171 10 min .0699 .0693 .0419 .0604 0.0160 Use the grid below to plot your data. Label the axes appropriately. If you are drawing your graph using excel remove this grid and insert it here. 0 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 1 2 3 4 5 6 7 8 9 10 Standard Deviation of the Reaction Rate Minutes Standard deviation of ONP 8. Do all time intervals provide equally reliable measurements of the reaction rate at 0.2 mM ONPG? Why or why not? A.I. Medrano, Ph.D. & D.L. Pattison, Ph.D. UH Department of Biology and Biochemistry. BIOL 1106 Lab