Week 11 laboratory reflection - Microscopy and Protista

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Indiana University, Bloomington *

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L113

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Biology

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Dec 6, 2023

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1 Name: Emma Manion Laboratory Reflection: Microscopy and Protista Instructions: Make sure to scroll through the entire document to answer all questions. It would also help with grading if you put your typed answers in a different (but readable) color font . Save and upload your completed assignment to the associated assignment page on Canvas. It is the student’s responsibility to confirm that the correct file was submitted to Canvas by the due date. 1. Answer the following questions on Microscopy: a. (2pts) Describe two ways a dissecting microscope is different from a compound microscope. The compound microscope has two sets of lenses (ocular and objective) white dissecting only has one (objective). The dissecting microscope has a lower overall magnification than the compound. b. (2pts) In future labs, you will be observing different phenotypes (i.e. external physical characteristics) of live Drosophila (fruit flies). Explain one reason why a dissecting microscope is appropriate for this task and a second (different) reason why a compound microscope is not appropriate for this task. The dissecting gives a greater working space between what you are observing and the objective. This will allow for the phenotype to be easier to be seen. The compound may be too magnified. The dissecting microscope allows you to see all external features with low magnification and a 3D view. This is ideal when looking at the phenotype. c. (1pts) Explain why you should you always begin on the lowest objective lens when using the compound microscope. This allows for more of the object to be fully seen and helps locate the part of it one wants to see. 2. Answer the following questions reflecting on organisms in the group Protista: a. (3pts) In lab, you observed three different groups of Protozoans that are classified based on their mode of locomotion. List these three groups and describe how each group moves. You should provide more than just name of the structure they use to move. To review and further observe these 3 locomotion strategies, you can watch the following video of a scientist’s microscopy footage : https://youtu.be/PPls7CQxOBA , from ~3:27 to 6:15. The 3 groups were ciliate, flagella, and amoeba. Flagella protozoans move with a whip-like structure, ciliates move using hair-like projections, and amoeba move by using bulging parts called pseudopodia (false feet).

2 b. (2pts) Describe two things about Protists (besides locomotion) that you learned about or observed in this lab. I learned that organisms are unicellular. While these organisms do not look complex, the modes in which they function (move, reproduce, and obtain energy) are all carried out by one cell. This differs from multicellular because these use many different cells to function. The life span of unicellular organisms is short due to this “ high workload ” .

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