Lab 4 Assignment Template

docx

School

Carleton University *

*We aren’t endorsed by this school

Course

1103

Subject

Biology

Date

Dec 6, 2023

Type

docx

Pages

Uploaded by MateWillpowerTarsier42

Fall 2023 Lab 4 Experimental – Epithelial Cells & DNA Extraction BIOL 1103 Lab 4 Assignment – Examining Epithelial Cells & DNA Extraction Full Name: ____________________ Student #: ________________ Lab section: __________ Although the experimental work will be completed individually and in pairs, all work submitted must be completed individually. It is important that you do not copy from the lab manual or from each other. DO NOT plagiarize! Unless otherwise specified in the question, all answers should be written using complete sentences, paragraph style. Part I: Examination of Human Epithelial Cells 1. Using complete sentences, write a detailed description of the general characteristics of one of the cheek epithelial cells that you observed (4 marks) . 2. Using the space provided below, create a relatively detailed diagram of the cheek cell you described in Question 1. Be sure to include a proper figure title, diagram magnification, and label visible structures (3 marks) . 1

Fall 2023 Lab 4 Experimental – Epithelial Cells & DNA Extraction BIOL 1103 3. In the space below, calculate the actual size of a cheek cell and its nucleus, along with the magnification of your diagram (Question 2) relative to the actual cheek cell size. Show all calculations and units. Hint: Refer to Lab 1 background information for calculating actual size and magnification for a scientific drawing (6 marks) . Cheek Cell Size Nucleus Size Drawing Magnification 4. Think back to Lab 1, where you examined microorganisms in samples of river water. What are two noticeable differences between the epithelial cells you have just observed and the organisms you observed in Lab 1 (2 marks) ? Part II: DNA Extraction and Precipitation of Saliva 2

Fall 2023 Lab 4 Experimental – Epithelial Cells & DNA Extraction BIOL 1103 5. What is the main purpose of adding soap into your solution? What effect does it have on the epithelial cells (2 marks) ? 6. What is the purpose of adding the salt into your solution? What are its effects on the epithelial cells (2 marks) ? 7. Why is cold isopropyl alcohol added into the soap and salt solution (1 mark) ? 8. Using complete sentences, write a detailed description of what transpired after adding the cold alcohol into your test tube (2 marks) . Part III: DNA in a Microscope 9. Using complete sentences, write a detailed description of the substance extracted from your test tube and observed under the microscope (3 marks) . 10. DNA is approximately 2 nm in diameter and is not resolvable as individual DNA molecules using the microscopes used in the first-year laboratories. Yet in this lab, you examined a precipitated substance in the microscope. 3

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Fall 2023 Lab 4 Experimental – Epithelial Cells & DNA Extraction BIOL 1103 a. Using the ocular micrometer, determine the approximate size of the strands within the precipitate. If your sample was clumped, use your best approximation. Show your work (2 marks) . b. What do you think the precipitate consists of (2 marks) ? c. How could you further process the precipitate to isolate it for DNA (2 marks) ? This assignment is due 1 week after completing your Lab 4 session, but must be submitted to Brightspace on the hour prior to your next session (i.e., 8 AM or 1 PM, depending on your section). IMPORTANT : Tutorial 4 will be held in TB 202 starting the week of Nov. 20 (Group 1) and Nov. 27 (Group 2). Refer to schedule in Brightspace or ask the lab coordinators if you have any questions. 4

Related Documents

Week 12 Worksheet.docx

Lab 11 Patterns of Inheritance.docx

Week 8 Lab 9 Photosynthesis.docx

Week 2 Worksheet (2).docx

Week 7 Lab 8 Cellular Respiration.docx

Week 6 Worksheet.docx

Lab 1 Assignment Template (1).pdf

Tutorial 4 Assignment Template.docx

UDN Project.pptx

Week 6 Lab 6 Cell Structure and Function.docx

Identical Twins and Intelligence 3.docx

unknown bacteria report.docx

Recommended textbooks for you

Comprehensive Medical Terminology

Nursing

ISBN:9781133478850

Author:Jones

Publisher:Cengage

An Illustrated Guide To Vet Med Term

Biology

ISBN:9781305465763

Author:ROMICH

Publisher:Cengage

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Body Structures & Functions Updated

Biology

ISBN:9780357191606

Author:Scott

Publisher:Cengage

Body Structures & Functions

Biology

ISBN:9781285695495

Author:Scott

Publisher:Cengage

Essentials Health Info Management Principles/Prac...

Health & Nutrition

ISBN:9780357191651

Author:Bowie

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
An Illustrated Guide To Vet Med Term
Biology
ISBN:9781305465763
Author:ROMICH
Publisher:Cengage
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Body Structures & Functions Updated
Biology
ISBN:9780357191606
Author:Scott
Publisher:Cengage
Body Structures & Functions
Biology
ISBN:9781285695495
Author:Scott
Publisher:Cengage
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage