Antibioticresistancecasestudyworksheet

NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCE Antibiotic Resistance: Can We Ever Win? By Maureen Leonard, Biology Department, Mount Mary College, Milwaukee, WI Part I – Measuring Resistance Exercise 1 Plate S. aureus MRSA 1 PE 15 mm 0 mm ME 6 mm 0 mm CE 12 mm 0 mm VA 9 mm 13mm 2 PE 14 mm 0 mm ME 11 mm 0 mm CE 14 mm 0 mm VA 11 mm 12 mm 3 PE 18 mm 0 mm ME 8.5 mm 0 mm CE 20 mm 0 mm VA 6 mm 9 mm Exercise 2 S. aureus MRSA Average SE Average PE 15.7 1.2 0 ME 8.5 1.45 0 CE 15.3 2.4 0 VA 8.67 1.46 11.3 Exercise 3 Redraw Table 1 and 2 into a single, more organized table. Be sure to label the table appropriately. Exercise 4 Graph the results from Table 2. Be sure to label the figure and axes correctly.

Answer the following questions Questions 1. What do you think the experimental question is? ● I think the experimental question is which antibiotic is more effective against staph & MRSA. 2. What hypotheses can you come up with to answer the experimental question? ● a hypothesis that I can come up with to answer the experimental question would be penicillin penicillin would be the most effective antibiotic staph. while Vancomycin will be the most effective against MRSA. 3. If your hypothesis is correct, what would the plates look like (i.e., what predictions would you make for each hypothesis)? ● The plates for staphylococcus aureus would have the largest inhibition zones around penicillin, and the plates for MRSA would have the largest inhibition zone around vancomycin. 4. Is the experiment you just collected data for an appropriate test of the experimental question you came up with in your answer to Question 1? 5. Which antibiotics where most effective against S. aureus? Against MRSA? ● penicillin and vancomycin 6. When comparing the antibiotics effective against both, were there differences in effectiveness? ● Yes, there’s effectiveness was different, MRSA demonstrated resistance to the most effective antibiotic against Aureus 7. What other questions do the data shown in Figure 1 make you think of? ● Why is penicillin more effective? Part II – Resistance Questions

6. Does Table 2 change your interpretation of the experimental data from Question 4? Why or why not? ● No table number two does not change my interpretation of the experimental data from question four because there is still a large decrease in MRSA cells in those treated with the FTsZ inhibitor and Imipenem combined. 7. How eff ective was the FtsZ inhibitor alone? Imipenem alone? ● Both FTsZ and Imipenem are not effective alone but when they are used together they can be very effective. 8. How eff ective was the combination of the inhibitor and the -lactam antibiotic? ● when the inhibitors in the lactam antibiotic are combined and the antibacterial was higher than when used separately. 9. How would you explain these results? ● The results, so that combining drugs has a greater antibacterial or synergistic impact. 10. What questions would you pursue next? ●