PhysioEx Exercise 8 Activity 1

PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 1: Assessing Starch Digestion by Salivary Amylase Name: Joseph Ramirez Date: 16 November 2023 Session ID: session-c6e10698-3d5f-4f36-950a-4dfc52e0d457 Pre-lab Quiz Results You scored 100% by answering 6 out of 6 questions correctly. Experiment Results The substrate for amylase is You correctly answered: starch and carbohydrate. 1 Which of the following is true of enzymes? You correctly answered: Their activity can be a ff ected by temperature and pH. 2 The reagent IKI tests for the presence of You correctly answered: starch. 3 Which of the following is not true of controls? You correctly answered: A negative result with a positive control is required to validate the test. 4 Which of the following is an end product of starch digestion? You correctly answered: maltose and glucose. 5 Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of . You correctly answered: water. 6

Predict Question Stop & Think Question Experiment Data Tube № Reagent 1 Reagent 2 Reagent 3 Treatment Time Temp IKI Benedict's 1 pH 7.0 Bu ff er Starch Amylase Boiled 60 37 + - 2 Amylase Starch pH 7.0 Bu ff er Frozen 60 37 - ++ 3 Amylase Starch pH 7.0 Bu ff er None 60 37 - ++ 4 Amylase Deionized Water pH 7.0 Bu ff er None 60 37 - - 5 Deionized Water Starch pH 7.0 Bu ff er None 60 37 + - 6 Deionized Water Maltose pH 7.0 Bu ff er None 60 37 - ++ 7 Amylase Starch pH 7.0 Bu ff er None 60 37 - ++ 8 Amylase Starch pH 9.0 Bu ff er None 60 37 + + Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Predict Question: What e ff ect do you think boiling and freezing will have on the activity of the amylase enzyme? Your answer: Boiling will decrease amylase activity and freezing will have no e ff ect. 1 Tube 2 (amylase, starch, pH 7.0 bu ff er) appears to have the same amount of starch digested as tube 3 (amylase, starch, pH 7.0 bu ff er) because You correctly answered: freezing had no e ff ect on the enzyme. 1 Tubes 3 (amylase, starch, pH 7.0 bu ff er), 7 (amylase, starch, pH 2.0 bu ff er), and 1

Your answer: Amylase was most active at a pH of 7.0. This is signi fi cant because a pH of 7.0 is the same pH of the mouth. Brie fl y describe the need for controls and give an example used in this activity. Your answer: Controls are needed so they can be compared to the changes made to tubes. It shows the results of the experiment and justify them. An example in the experiment would be test tube 5, because the enzyme that detects contaminated glucose is absent. 4 Describe the signi fi cance of using a 37°C incubation temperature to test salivary amylase activity. Your answer: Using a incubation temperature of 37 degrees celsius to test salivary amylase activity is signi fi cant because it is the same temperature of the body, showing that it is ideal for amylase. 5