Penn foster externship 2 skill 24

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Penn Foster College *

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Course

110

Subject

Biology

Date

Dec 6, 2023

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docx

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2

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Penn foster externship 2 skill 24 On November 3 rd , 2023, MACY was scheduled to have an aural hematoma repaired surgically, and at that time I performed a buccal mucosal bleeding time test on her. First, I put on gloves, and then I began to perform the BMBT. Her lip was folded up and secured with a gauze tie to expose the oral mucosa. I made sure to visualize where the premolars are while the lip was folded up because my incision will be made at the level of the premolars. I took my lancet and removed the safety clip and placed it against the lip above the premolars. I released the lancet to make a 1mm incision. I started a timer and then began to blot the blood with a gauze square and allowed the blood to be absorbed into the gauze square. I blotted every 5 seconds until a clot formed and the bleeding stopped. The bleeding stopped after 2 minutes and 25 seconds. Normal time range is between 1 minute and 5 minutes. Crossmatching is performed to make sure that the donor blood will work in the recipient patient. There is a two-part procedure for crossmatching, major and minor crossmatching. They both require a serum sample and a whole blood sample. For major crossmatching, a few drops of serum from the recipient sample are added to the donor sample, after the donor RBCs are washed 3 times in a saline solution. The mixture is incubated and then centrifuged. The macroscopic or microscopic presence of hemolysis or agglutination indicates a blood-type mismatch (Sirois, M. (2020). Laboratory Procedures for Veterinary Technicians . (pp. 119-120). Elsevier). For minor crossmatching, a few drops of serum from the donor sample are added to the washed receipt sample. This mixture is incubated and then centrifuged. Both tests are needed before any blood transfusion to ensure there are no reactions. Some complications of transfusions include infection from contaminated blood, a decrease in blood calcium levels and accumulation of fluid in the
lungs (Cotter, S. DVM. (December 2017). Blood Groups and Blood Transfusions in Dogs. Merck Veterinary Manual).
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