Post LAB Experiment 2 Biochem (2)

Solution Absorbance mg/ml aspirin Standard solution  -  1.6 mg/mL A  0.638  0.08 mg/mL B 0.504   0.064 mg/mL C 0.376  0.048 mg/mL  D 0.259   0.032 mg/mL E 0.126  0.016 mg/mL  A = -log T  where T =  %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated    Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg                            Absorbance = 0.45 Student prepared tablet from practical 5                           Absorbance = 0.19   Using the data provided, the graph you have generated, and the procedure that was used…

10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944

Rx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMES

Benzalkonium chloride (E=0.16) 1.5g qs 100 ml Distilled water 0.9% Nacl solution ad Make isotonic solution Questions: 1. How many ml of distilled water must be added to make the Benzalkonium chloride preparation isotonic? 2. How many ml of 0.9% NaCl must be added to make a 100ml solution?

Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left off of a 15-mL bottle of the tincture, and the ethyl alcohol evaporates leaving a final volume of 9.5 mL, what is the concentration of thimerosal in the evaporated solution expressed as a ratio strength?

Calculate the percentage of stated content in paracetamol tablets using the calbration curve given above and the following data: Data • Weight of 20 tablets¼12.2243 g • Weight of tablet powder taken¼152.5 mg • Stated content per tablet¼500 mg • Initial extraction volume¼200 ml. Dilution steps • 20 ml into 100 ml • 10 ml into 100 ml • Area of chromatographic peak for paracetamol extracted from the tablets¼44 519.

How many mLs should be removed from your dextrose 70% bag?

A sample of yeast extract has been analyzed of its invertase activity. The effect of temperature on the invertase activity was monitored using the same dinitrosalicylic acid assay used in your experiment in the laboratory. Refer to the data below. What is the amount of sucrose hydrolyzed (in mg/mL) at optimum temperature ?

Penicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.

Discuss the suitability of bromocresol green and thymol blue (2nd change only) for use as indicators in an acetic acid - sodium hydroxide titration. Paragraph в I A + v ...

11 of 11 What volume (in µL to 1 decimal place) of stock SybrSafe solution (3.4 mg/mL) would you add to 120 mL of agarose solution to give a final concentration of 0.4 pg/mL? Answer: Submit < Previous Question Assessment Navigator Questionmark Ondemand licensed to University of Dundee DII DD 80 000 000 F10 F8 F9 F7 esc F5 F6 F3 F4 F1 F2 24 ... 6 7 * 00

Note: total volume is 94 ml

how many grams of sr(no2)2 are needed to make 200 ml of 0.300M solution of sr(no2)2

help please with calculations

Rf Sample values 0.21 В 0.39 C 0.33 0.27 Four amino acid samples were subjected to a normal-phase chromatography set up yielded the following Rf values. Which among the samples is the least polar? E and O A O B OD

A 10.00-mL aliquot of unknown oxalic acid solution required 15.44 mL of 0.011 17 M KMnO4 solution to reach the purple end point. A blank titration of 10 mL of similar solution containing no oxalic acid required 0.04 mL to exhibit detectable color. Find the concentration of oxalic acid in the unknown.

Tirofiban (C22H36N2O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose solution and adding 50 mL of concentrated solution. Sofia who weighs weighs 87 kg requires a tirofiban dose of 0.1 mcg/kg/min for 12 to 24 hours. What would the infusion rate be (mL/h)? (Answer to 1 decimal place.)

2

You need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.

Protein concentration standards 0.6 0.5 0.4 0.3 0.2 0.1 0.5 1.5 2.5 Albumin concentration (mg/mL) A standard curve of known concentrations of protein were reacted with the Biuret reagent. A linear curve fit is presented above. • Use the graph to estimate the concentration of an unknown sample with an A550 value of 0.416 (A rough estimate is fine. You do not need a calculator for this problem) • Use the graph to estimate the concentration of an unknown sample with an A550 value of 0.233 • Which instrument did we use to measure the intensity of the color of proteins that reacted with Biuret reagent?

9. 3 μL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Substrate volume in mL Dilution factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL

Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate (C22H36N2O5S.HCI.H₂O MWt=495.1) at 0.281 mg/mL in a concentrated solution. A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose solution and adding 50 mL of concentrated solution. Sofia who weighs weighs 87 kg requires a tirofiban dose of 0.1 mcg/kg/min for 12 to 24 hours. What would the infusion rate be (mL/h)? (Answer to 1 decimal place.) Answer:

Table of caffeine standards concentration Sample Conc, ppm Std1 16 Std2 32 Std3 48 Std4 64 Std5 80 If the volume used to make 100 mL  of std 1 is 2 uL what is the concentration in M used to make a standard calibration curve?  The standards are going to be used to build calibration curve to analyze caffeine in  an energy drink. If 500 mL of  the energy drink  has target of 400 mg  caffeine, how will you prepare the sample if you need 10 mL for the analysis ? .  Caffeine MM=194.19 g/mol.

A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).

Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose s and adding 50 mL of concentrated solution. Sonya who weighs weighs 60 kg requires a tirofiban dose of 0.4 mcg/kg/min for 30 minute- What volume would remain after 30 minutes? Answer: 94.53 mL X The correct answer is: 236 mL

You just made a 1.5M permanganate solution. What concentration is your potassium permanganate solution in percent? "K: 39.10 g/mole" "Mn: 54.94 g/mole" "O: 16 g/mole" "MW of KMnO4 is 158.04 g/mole" O a. 0.24% Ob. 20.1% O c. 15.8% O d. 23.7% O e. 2%