Pearson eText Biology: Life on Earth with Physiology -- Instant Access (Pearson+)
12th Edition
ISBN: 9780135755785
Author: Gerald Audesirk, Teresa Audesirk
Publisher: PEARSON+
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Chapter 4.3, Problem 1CYL
- describe the structure and features shared by all cells?
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Referring to the Standard Genetic Code table, categorize the chemical properties of each of the 24 amino acids that make up the ER Signal Peptide (hydrophobic, hydrophilic, positive charge, or negative charge). What is notable about the chemical properties of the amino acids that make up the ER Signal Peptide?
methionine- translation code
tra-hyperopic amino acid
R Origine eukiauk aicd amino
glutamine HYDOICO ACIDS
gaac -CHANGED AMINO ACID
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
a) The relationship between the Hawaiian bobtail squid and V. fischeri is symbiotic where both species benefit. What is the benefit to each?
b) Why might quorum sensing be beneficial to pathogenic bacteria?
c) How might scientists use quorum sensing to treat bacterial infections?
Chapter 4 Solutions
Pearson eText Biology: Life on Earth with Physiology -- Instant Access (Pearson+)
Ch. 4.1 - trace the historical development of the cell...Ch. 4.1 - list the three principles of the cell theory?Ch. 4.2 - Prob. 1TCCh. 4.2 - Prob. 1CYLCh. 4.2 - Prob. 2CYLCh. 4.3 - Prob. 1CSCCh. 4.3 - describe the structure and features shared by all...Ch. 4.3 - distinguish prokaryotic from eukaryotic cells?Ch. 4.3 - Prob. 3CYLCh. 4.4 - describe the structure and function of the major...
Ch. 4.4 - Prob. 2CYLCh. 4.5 - Prob. 1CSCCh. 4.5 - Why do the chromosomes in chromatin condense in...Ch. 4.5 - Using Fig. E4 4. plot the changes in each country...Ch. 4.5 - Why is it advantageous for all cellular membranes...Ch. 4.5 - Why is it important for lysosomal enzymes to be...Ch. 4.5 - Over the years, scientists have wondered how many...Ch. 4.5 - What problems would arise if the trachea were...Ch. 4.5 - Prob. 1CYLCh. 4.5 - list the structures found in animal but not plant...Ch. 4.5 - describe the structure and function of each major...Ch. 4.5 - Prob. 1CTCh. 4 - Which of the following is/are found only in...Ch. 4 - Which of the following is not a function of the...Ch. 4 - Prob. 3MCCh. 4 - Prob. 4MCCh. 4 - Prob. 5MCCh. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Prob. 4FIBCh. 4 - Prob. 5FIBCh. 4 - Two organelles that are believed to have evolved...Ch. 4 - Prob. 7FIBCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Describe the nucleus and the function of each of...Ch. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Describe the structure and function of the...Ch. 4 - Prob. 8RQCh. 4 - Prob. 9RQCh. 4 - List the structures of bacterial cells that have...Ch. 4 - Prob. 1ACCh. 4 - Prob. 2ACCh. 4 - What problems would an enormous round cell...
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
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