Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 3, Problem 14Q
The results of your patient’s wound culture just arrived, and
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You have a mixed culture of 2 different bacterial species. One of the species is Gram negative bacilli and the other species is Gram positive cocci. You have noticed that one species is killed by penicillin antibiotics (attacks peptidoglycan) while the other survives. Neither species has antibiotic resistance genes. How can you explain this result? Remember to make direct reference to each bacterial species in your response
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is one experiment you could do, involving culturing the organism?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is a procedure you could do, involving making a slide of the organism?
Chapter 3 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 3.1 - List the structures all bacteria possess.Ch. 3.1 - Identify three structures some but not all...Ch. 3.1 - Describe three major shapes of bacteria.Ch. 3.1 - Provide at least four terms to describe bacterial...Ch. 3.2 - Describe the structure and function of six...Ch. 3.2 - Prob. 6AYPCh. 3.2 - Q. Device-associated infections are very common...Ch. 3.3 - Differentiate between the two main types of...Ch. 3.3 - Prob. 8AYPCh. 3.3 - Prob. 9AYP
Ch. 3.3 - Prob. 2MMCh. 3.4 - Identify seven structures that may be contained in...Ch. 3.4 - Prob. 11AYPCh. 3.4 - Prob. 1NPCh. 3.5 - Compare and contrast the major features of...Ch. 3.6 - Differentiate between Bergeys Manual of Systematic...Ch. 3.6 - Name four divisions ending in cutes and describe...Ch. 3.6 - Define a species in terms of bacteria.Ch. 3 - Archaea a. are most genetically related to...Ch. 3 - Prob. 2QCh. 3 - Suppose an argument in your city has erupted about...Ch. 3 - Prob. 4QCh. 3 - As a supervisor in the infection control unit, you...Ch. 3 - Prob. 6QCh. 3 - Prob. 7QCh. 3 - Prob. 8QCh. 3 - Bacteria and archaea have a much greater diversity...Ch. 3 - Prob. 10QCh. 3 - Bacteria have been found to change the structures...Ch. 3 - Bacterial and archaeal chromosomes are not...Ch. 3 - Prob. 13QCh. 3 - The results of your patients wound culture just...Ch. 3 - We know that bacteria/archaea and their genetics...Ch. 3 - Find the true statement about biofilms. a. They...Ch. 3 - Suggest more than one reason why bacteria may...Ch. 3 - Construct arguments agreeing with and refuting...Ch. 3 - Which of the following would be used to identify...Ch. 3 - During the cold war between the Soviet Union and...Ch. 3 - During the cold war between the Soviet Union and...Ch. 3 - From chapter 2, figure 2.18. Explain why some...
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- A hospital lab technician working with a particular strain of streptococci culture on blood agar plates suddenly came down with a case of sore throat. Using Koch's Postulates as a guide, develop a step by step experimental protocol to determine if the organism growing on the plates was in fact the cause of the technician's sore throat.arrow_forwardA farmer working on a piece of machinery gets his shirtsleeve caught in a moving piece of the equipment. His shirt is sliced, and a sharp blade covered in mud slices through his upper arm. He attempts to control the bleeding and immediately seeks medical attention. After 3 days, he develops a fever and his arm becomes extremely swollen and painful. Pulling back the bandages, he finds that the wound has become blackened and is leaking bloody fluid. Microscopic analysis of the fluid reveals the presence of gram-positive bacilli. a. Discuss what condition the patient is suffering from and the likely causative agent of this infection. b. Explain how the patient contracted this pathogenic microbe and what virulence factors contributed to the pathogenesis seen at the wound site. c. In addition to antibiotics, the physician prescribes hyperbaric therapy. Describe what this treatment involves and how it could be therapeutic to this patient.arrow_forwardThe figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.arrow_forward
- A patient is experiencing high fever and body aches from an infected skin wound. You suspect a bacterial infection and perform a Gram stain from a pustular exudate why are you able to rule out an ENDOTOXIN as the cause of the fever and from the image, which organism do you suspect? The bacteria is purple(+).arrow_forwardYou are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskarrow_forwardYou mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) Falsearrow_forward
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