Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 28.2, Problem 6AYLO
Ovarian and hormonal changes that bring on female climacteric and menopause; effects commonly experienced by perimenopausal women; and differences between female and male climacteric
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 28 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 28.1 - Prob. 1BYGOCh. 28.1 - How is the structure of the uterine tube mucosa...Ch. 28.1 - Prob. 3BYGOCh. 28.1 - Prob. 4BYGOCh. 28.1 - Prob. 1AYLOCh. 28.1 - What mature female structures arise from the...Ch. 28.1 - Prob. 3AYLOCh. 28.1 - General structure of ovarian follicles; their...Ch. 28.1 - Prob. 5AYLOCh. 28.1 - Prob. 6AYLO
Ch. 28.1 - Prob. 7AYLOCh. 28.1 - The tilt of the vagina and its relationship to...Ch. 28.1 - Prob. 9AYLOCh. 28.1 - Prob. 10AYLOCh. 28.1 - The prevalence of breast cancer; its diagnostic...Ch. 28.2 - Prob. 5BYGOCh. 28.2 - Prob. 6BYGOCh. 28.2 - Prob. 7BYGOCh. 28.2 - Prob. 1AYLOCh. 28.2 - Prob. 2AYLOCh. 28.2 - Thelarche, pubarche, and menarche as signs of...Ch. 28.2 - Prob. 4AYLOCh. 28.2 - Prob. 5AYLOCh. 28.2 - Ovarian and hormonal changes that bring on female...Ch. 28.2 - Prob. 7AYLOCh. 28.3 - Prob. 8BYGOCh. 28.3 - Prob. 9BYGOCh. 28.3 - Prob. 10BYGOCh. 28.3 - Describe what happens in the uterus during the...Ch. 28.3 - Describe the effects of FSH and LH on the ovary.Ch. 28.3 - Prob. 13BYGOCh. 28.3 - Prob. 1AYLOCh. 28.3 - Prob. 2AYLOCh. 28.3 - Prob. 3AYLOCh. 28.3 - Ways in winch meiosis in the female differs from...Ch. 28.3 - How far meiosis has progressed by the time the egg...Ch. 28.3 - Prob. 6AYLOCh. 28.3 - Prob. 7AYLOCh. 28.3 - Prob. 8AYLOCh. 28.3 - Prob. 9AYLOCh. 28.3 - Prob. 10AYLOCh. 28.3 - Prob. 11AYLOCh. 28.3 - Production, structure, function, and eventual...Ch. 28.3 - Four phases of the menstrual cycle; what happens...Ch. 28.4 - Prob. 14BYGOCh. 28.4 - Prob. 15BYGOCh. 28.4 - Prob. 16BYGOCh. 28.4 - Prob. 1AYLOCh. 28.4 - Physiological responses of orgasm and resolution...Ch. 28.5 - List the roles of HCG, estrogen, progesterone, and...Ch. 28.5 - Prob. 18BYGOCh. 28.5 - Prob. 19BYGOCh. 28.5 - Prob. 20BYGOCh. 28.5 - Prob. 21BYGOCh. 28.5 - Prob. 22BYGOCh. 28.5 - Prob. 1AYLOCh. 28.5 - Prob. 2AYLOCh. 28.5 - Prob. 3AYLOCh. 28.5 - Prob. 4AYLOCh. 28.5 - Prob. 5AYLOCh. 28.5 - Causes of morning sickness, constipation, and...Ch. 28.5 - Prob. 7AYLOCh. 28.5 - Prob. 8AYLOCh. 28.6 - Prob. 23BYGOCh. 28.6 - Prob. 24BYGOCh. 28.6 - Prob. 25BYGOCh. 28.6 - Prob. 26BYGOCh. 28.6 - Prob. 27BYGO
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