Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 22, Problem 3MCQ
Gastric ulcers are caused by
- a. Treponema vincentii.
- b. Prevotella intermedia.
- c. Helicobacter pylori.
- d. all of the above.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 22 Solutions
Microbiology: A Systems Approach
Ch. 22.1 - Prob. 1AYPCh. 22.1 - Prob. 2AYPCh. 22.2 - Prob. 3AYPCh. 22.2 - Prob. 4AYPCh. 22.3 - Prob. 2CFCh. 22.3 - List the possible causative agents for the...Ch. 22.3 - Prob. 6AYPCh. 22.3 - Name one distinct feature for each of the acute...Ch. 22.3 - Prob. 8AYPCh. 22.3 - Prob. 9AYP
Ch. 22.3 - Differentiate among the main types of hepatitis...Ch. 22.4 - Prob. 11AYPCh. 22.4 - Prob. 12AYPCh. 22.4 - Prob. 13AYPCh. 22.4 - Prob. 14AYPCh. 22.4 - Describe the type of disease caused by Trichinella...Ch. 22.4 - Prob. 16AYPCh. 22 - Prob. 1CFCh. 22 - Food moves down the GI tract through the action of...Ch. 22 - Prob. 2MCQCh. 22 - Gastric ulcers are caused by a. Treponema...Ch. 22 - Prob. 4MCQCh. 22 - Prob. 5MCQCh. 22 - Prob. 6MCQCh. 22 - This microorganism is commonly associated with...Ch. 22 - Prob. 8MCQCh. 22 - Prob. 9MCQCh. 22 - Prob. 10MCQCh. 22 - Prob. 11TFCh. 22 - Giardia lamblia is a water-borne, flagellated...Ch. 22 - Prob. 13TFCh. 22 - Prob. 14TFCh. 22 - Enterobius vermicularis commonly known as the...Ch. 22 - Prob. 1CTQCh. 22 - Prob. 2CTQCh. 22 - Prob. 3CTQCh. 22 - Prob. 4CTQCh. 22 - Prob. 5CTQCh. 22 - Prob. 6CTQCh. 22 - Prob. 7CTQCh. 22 - a. Describe methods used to definitively diagnose...Ch. 22 - Prob. 9CTQCh. 22 - Prob. 10CTQCh. 22 - Prob. 1CCCh. 22 - Prob. 2CCCh. 22 - Prob. 3CCCh. 22 - Prob. 4CCCh. 22 - Prob. 5CCCh. 22 - Prob. 6CCCh. 22 - Prob. 7CCCh. 22 - Prob. 8CCCh. 22 - Prob. 1VCCh. 22 - Prob. 2VCCh. 22 - Prob. 1CM
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