Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them. a. Find the clone with the gene for lactase. b. Insert the plasmids into bacteria and grow the bacteria into clones. c. Isolate the gene for lactase. d. Create recombinant plasmids, including one that carries the gene for lactase.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

If using animals in medical experiments could save human lives, is it ethical to do so? In your answer, apply at least one ethical theory in support of your position.

You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?

You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.

Answer 2

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 8

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer 12

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Answer 13

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 14

Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - In making recombinant DNA, what is the benefit of...Ch. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - Prob. 6SQ Ch. 12 - After a gel electrophoresis procedure is run, the...Ch. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Prob. 9SQ Ch. 12 - Some scientists once joked that when the DNA...

Concept explainers

Videos

Answer to Problem 1SQ

Explanation of Solution

Want to see more full solutions like this?

Chapter 12 Solutions

Additional Science Textbook Solutions