Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 11, Problem 4FIB
Genes that are present on one sex chromosome but not the other are called _________.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 11 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 11.1 - describe the relationships among chromosomes, DNA,...Ch. 11.1 - Prob. 2CYLCh. 11.2 - distinguish between self-fertilization and...Ch. 11.2 - Prob. 2CYLCh. 11.3 - Sudden Death on the court Many traits, in humans...Ch. 11.3 - describe the pattern of inheritance of a trait...Ch. 11.3 - Prob. 1TCCh. 11.3 - Prob. 2CYLCh. 11.3 - Prob. 3CYLCh. 11.4 - Prob. 1CYL
Ch. 11.4 - Prob. 1TCCh. 11.4 - Prob. 2CYLCh. 11.4 - Prob. 2TCCh. 11.4 - Prob. 3CYLCh. 11.5 - Prob. 1CSCCh. 11.5 - Prob. 1CYLCh. 11.5 - Prob. 1HYEWCh. 11.5 - Prob. 1TCCh. 11.5 - explain how polygenic inheritance and...Ch. 11.6 - describe how the patterns of inheritance differ...Ch. 11.7 - Prob. 1CYLCh. 11.7 - explain why most sex-linked traits are controlled...Ch. 11.7 - describe the pattern of inheritance of sex-linked...Ch. 11.8 - Prob. 1CSRCh. 11.8 - use pedigrees to determine the pattern of...Ch. 11.8 - Prob. 1ETCh. 11.8 - Prob. 1TCCh. 11.8 - Prob. 2CYLCh. 11.8 - Prob. 2ETCh. 11 - Prob. 1ACCh. 11 - An organism is described as Rr, with red coloring....Ch. 11 - In certain cattle, hair color can be red...Ch. 11 - Prob. 1MCCh. 11 - Define the following terms: gene, allele,...Ch. 11 - Prob. 2ACCh. 11 - Prob. 2FIBCh. 11 - Prob. 2GPCh. 11 - Prob. 2MCCh. 11 - Prob. 2RQCh. 11 - Prob. 3FIBCh. 11 - Prob. 3GPCh. 11 - Prob. 3MCCh. 11 - Prob. 3RQCh. 11 - Genes that are present on one sex chromosome but...Ch. 11 - Prob. 4GPCh. 11 - Prob. 4MCCh. 11 - Prob. 4RQCh. 11 - Prob. 5FIBCh. 11 - Prob. 5GPCh. 11 - Prob. 5MCCh. 11 - Prob. 5RQCh. 11 - Prob. 6GPCh. 11 - Prob. 6RQCh. 11 - In the couple described in Problem 6, the woman...
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