Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

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Answer 1

Textbook Question

Chapter 10, Problem 1NST

Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

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Answer 2

Textbook Question

Chapter 10, Problem 1NST

Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

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Answer 3

Textbook Question

Chapter 10, Problem 1NST

Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

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Answer 4

Textbook Question

Chapter 10, Problem 1NST

Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

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Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

Answer 8

Expert Solution & Answer

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Answer 12

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Answer 13

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

Answer 14

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Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

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