Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 1, Problem 6Q
Imagine a way you might design a drug to destroy microbes that will not harm human cells.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 1 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 1.1 - List the various types of microorganisms that can...Ch. 1.1 - Describe the role and impact of microbes on the...Ch. 1.1 - Explain the theory of evolution and why it is...Ch. 1.1 - Explain the ways that humans manipulate organisms...Ch. 1.1 - Summarize the relative burden of human disease...Ch. 1.1 - Differentiate among bacteria, archaea, and...Ch. 1.1 - Identify an acellular infectious agent that is...Ch. 1.1 - Compare and contrast the relative sizes of the...Ch. 1.1 - Q.Can you think of a logical reason that a microbe...Ch. 1.1 - NCLEX PREP 1. For which of the following disease...
Ch. 1.2 - Make a time line of the development of...Ch. 1.2 - List some recent microbiology discoveries of great...Ch. 1.2 - Identify the important features of the scientific...Ch. 1.3 - Name the four main families of biochemicals.Ch. 1.3 - Provide examples of cell components made from each...Ch. 1.3 - Differentiate among primary, secondary, tertiary,...Ch. 1.3 - List the three components of a nucleotide.Ch. 1.3 - Name the three nitrogen bases of DNA and RNA.Ch. 1.3 - Prob. 17AYPCh. 1.3 - Recall three characteristics common to all cells.Ch. 1.3 - Q. Use context in the paragraph above to deduce...Ch. 1.3 - Prob. 2NPCh. 1.4 - Differentiate among the terms nomenclature,...Ch. 1.4 - Create a mnemonic device for remembering the...Ch. 1.4 - Correctly write the binomial name for a...Ch. 1.4 - Draw a diagram of the three major domains.Ch. 1.4 - Explain the difference between traditional and...Ch. 1 - Prob. 1QCh. 1 - Name six types of microorganisms that we are...Ch. 1 - Defend the argument that a web of life is a more...Ch. 1 - Which of the following is a macromolecule that...Ch. 1 - Prob. 5QCh. 1 - Imagine a way you might design a drug to destroy...Ch. 1 - Prob. 7QCh. 1 - Provide an argument about why metabolic...Ch. 1 - Provide a possible interpretation of the finding...Ch. 1 - DNA leads to RNA which can lead to the creation of...Ch. 1 - Compare and contrast the RNA molecule with the DNA...Ch. 1 - Suggest an argument for why eukaryotic cells have...Ch. 1 - Prob. 13QCh. 1 - Defend or refute this statement: Microbes intend...Ch. 1 - Coevolution is a term describing the influence...Ch. 1 - Which of the following processes can be the result...Ch. 1 - Speculate about why scientists believe there are...Ch. 1 - Prob. 18QCh. 1 - When a hypothesis has been thoroughly supported by...Ch. 1 - Defend the use of complicated-sounding names for...Ch. 1 - Identify the most important component of the...Ch. 1 - Figure 1.2 Look at the red bat (the time that...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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