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You take the PKA catalytic subunit, the regulatory subunit, and the dimerization domains and mix them in two different test tubes. One contains cAMP and one does not. You want to see if they formed a complex, so design an experiment that'd allow you if they did or did not, but you are not allowed to covalent modify any of the proteins before mixing them.

You take the PKA catalytic subunit, the regulatory subunit, and the dimerization domains and mix them in two different test tubes. One contains cAMP and one does not. You want to see if they formed a complex, so design an experiment that'd allow you if they did or did not, but you are not allowed to covalent modify any of the proteins before mixing them.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question: You take the PKA catalytic subunit, the regulatory subunit, and the dimerization domains and mix them in two different test tubes. One contains cAMP and one does not. You want to see if they formed a complex, so design an experiment that'd allow you if they did or did not, but you are not allowed to covalent modify any of the proteins before mixing them.
Transcribed Image Text:Question: You take the PKA catalytic subunit, the regulatory subunit, and the dimerization domains and mix them in two different test tubes. One contains cAMP and one does not. You want to see if they formed a complex, so design an experiment that'd allow you if they did or did not, but you are not allowed to covalent modify any of the proteins before mixing them.
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