What is the BEST glassware that could be used to hold the agar powder while weighing? After 24 hours, growth can be seen from your plates. If you want to put dyes on your sample, what rack is used?

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Ared blood cell is placed into each of the following solutions. Indicate whether crenation,…

A: There are three kinds of solutions hypotonic, hypertonic, and isotonic. At the point when the solute…

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: The part of the heart that relaxes when filling up with blood before ejection to the pulmonary…

A: Heart is the most important organ that pumps blood. It is divided into four compartments; right and…

Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…

A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…

Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…

A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: What are some methods to prevent contamination during streak plating a medium?

A: Streak plate method is one of the most important methods used by the laboratories to isolate single…

Q: Write an introduction related to culture media and isolation of pure culture technique (streak plate…

A: In microbiology, there are five I’s which represent five different techniques used in the…

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: Why is it important to first determine whether growth occurred in a different medium, such as starch…

A: Culture medium can be described as a liquid or gel medium which provides minerals and nutrients to…

Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…

A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…

Q: In your own words, explain how gram staining is done and how it works.

A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…

Q: Choose the 4 most correct steps from the list below for a simple positive stain and organize them…

A: The above question is related to the laboratory technique of bacterial staining. Staining is a…

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods

A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…

Q: The lab provides you with 25 mg/ 100 mL of BAP stock solution. You only need 1 mg of BAP in 180 mL…

A: A substance used for growing and developing microorganisms, cells or tissues in a laboratory setting…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…

A: Sterilization of following- petri dish - By using autoclave or by a series of steps that includes…

Q: Where should a label be written on an agar plate?

A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…

Q: What are the most commonly used methods in staining? why?

A: Introduction  Staining is a technique for enhancing contrast in material, usually on a tiny scale.…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…

A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…

A: PREPARATION OF SMEAR ( FLOWCHART)

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…

A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…

Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…

A: The chocolate agar and the blood agar media both have the almost same composition. The only…

Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: gel electrophoresis

A: Gel Electrophoresis is a simple , rapid and analytical technique which is used for separating the…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: What is the purpose of iodine in the Gram stain?   To act as a counterstain   None of the other…

A: There are various staining techniques that are used to stain different cellular structures. The Gram…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Gram staining is also known as Gram's method is a staining method for identifying and separating…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.