What are the chief differences between the Ninhydrin and Bradford assays for amino acid quantification? Why would one technique be preferred over the other?

Q: 23. What would cause compounds with similar structures to be separates on a TLC plate as a…

A: The detail explaination to the above question is given in step 2.

Q: Based on the chromatograms, Symmetry C18 (top) is less selective than Symmetry Shield RP18 (bottom)…

A: In general, Symmetry Shield RP18 columns feature Water patented embedded polar group technology that…

Q: None

A: Significance of the Mass ErrorThe mass error in ppm is a crucial parameter in mass spectrometry as…

Q: What is 2D gel electrophoresis

A: 2D gel electrophoresis is a technique for detection and separation of proteins. It is a combination…

Q: and all basic drugs using SPE with hydrophobic resin. ▪ A)True In an untargeted analysis by GC-MS,…

A: 1) False  In SPE technique ,one or more analyte  from liquid sample are isolated by extracting or…

Q: With a purple dye mixture and acetone as the solvent, a student conducts a TLC experiment on a…

A: TLC is an important chromatographic technique for the identification and separation of mixtures of…

Q: Does the presence of a benzene ring or carboxylic acid functional group in a substance affect the…

A: General concepts of TLC analysis

Q: Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

A: Buffer is a solution that tends to maintain pH of the solution when acid or base is added to it.…

Q: In Thin Layer Chromatography，why does the least polar of the analytes （biphenyl）have a higher Rf…

A:

Q: In gel chromatography, what determines the degree of species separation in a state?

A: Given   Gel chromatography  and the degree of species separation .

Q: Q22. You make a standard curve of BSA using the same method as in the first class and obtain the…

A: a) Slope of the line is y=0.055x b) 51uL of acid precipitation fraction c) Absorbance = 0.972 Find…

Q: Mass Spec IR 100 80 - 60- 40- 20 - ca 1229 219 34 2126 1706 29 760 21 1361 10 11 20 678 0.0+ 40 45…

A: In the given problem, the mass spectra and the IR spectra of a compound is given. From the mass…

Q: What is the chemical shift Splitting pattern  Integration from this HnMR

A: Spectra 1 : Low resolution Spectra  Spectra 2 : High resolution Spectra

Q: Adenine and caffeine have retention times of 15.50 and 17.63 min, respectively, on a 30.0-cm column.…

A:

Q: A new protein of an unknown structure ha graphy (gel filtration) reveals that the native absence of…

A: Given: weight of native protein - 100 kDa dithiotreitol - 50 kDa

Q: Q24) Consider the following data obtained from the size exclusion chromatography of polypropylene,…

A: This is question on molecular weight of polymer

Q: In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 4…

A: Retention factor is the ratio of distance travelled by the solute and the distance travelled by the…

Q: A protein consists of two types of peptide chains (A and B) with an unknown stoichiometry (AxBy).…

A: Given that: peak area of A = 500,000 peak area of B = 100,000 molecular mass of A =  25,000…

Q: What kind of sample cleanup does solid-phase extraction accomplish in the analysis of caffeine and…

A: Introduction: Extraction is the process of separation of the molecules. There are two types of…

Q: Why are collision cells a necessary addition to mass spectrometers that employ electrospray…

A: In mass spectrometers that employ electrospray ionization, a major challenge is how one can fragment…

Q: What is the AG" value for binding (not dissociation) for a compound binding a receptor 5x 10 M?…

A: Given Data:Kd (Dissociation constant) of a receptor = 5 x 10-7 MTo determine: value for a compound…

Q: This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka…

A: The Beer-Lambert Law states that the log of the ratio of the intensities is equal to the product of…

Q: What are the units of the retention factor (Rf)? Briefly explain your conclusion.

A: The correct answer about Retention factor is given below

Q: What is the purpose of SDS in SDS-PAGE? 1. To denature proteins O2. To stain proteins 3. To create a…

A: Purpose of SDS in SDS-PAGE?

Q: Explain how we would run a paper chromatography on an amino acid sample (explain the procedure or…

A: Paper chromatography is the chromatography based on liquid-liquid partition chromatography. It…

Q: Analysis of protein purity using two-dimensional electrophoresis could involve

A:

Q: What is the matrix effect? How may it affect the results in quantitative analysis?

A: Liquid chromatography- Mass spectrometry (LC-MS) is a very important separation technique.

Q: How does the retention time of a compound change with separation conditions? Is this change linear…

A: The interval between the injection of a sample and the detection of substances in that sample is…

Q: From the six analytes listed below, n-octanal, n-octane, 1-chlorooctane, n-octanol, n-heptane, and…

A: The objective of the question is to predict the retention order of six analytes: n-octanal,…

Q: how might chromatography be used to analyze the composition of the dye in a marker?

A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…

Q: How would you determine the concentration of BPA in an unknown sample if given this table and the…

A: First, we have to find the average corrected peak area of the three trials for the standard…

Q: Identify 4 peaks and annotate the IR of ethyl-4-aminobenzoate

A: We are given the IR spectra of ethyl-4-aminobenzoate

Q: A series of caffeine standards were prepared and scanned with an IR spectrophotometer yielding the…

A: The above graph to determine the percentage of caffeine in aqueous solution of sample and to prepare…

Q: During TLC, what is the stationary phase and what is the mobile phase?

A: Chromatography is a laboratory technique for the separation of a mixture into its components. It is…

Q: An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl-sepharose…

A: Ion exchange chromatography is an analytical technique for separation of proteins on the basis of…

Q: If the stationary phase and the compound being tested are both polar, will the retention factor (Rf)…

A: Given that both the stationary phase and the compound being tested are polar.