Samples came to your laboratory for analysis. You are asked to identify the differences between these examples. You also learned that in order to catch the difference, I first need to isolate the DNA of the samples. In addition, the possibilities are super ..., chemicals, spectrophotometer, the best centrifuge… so everything is super .. You also isolated the DNA. It's time for the next step. There are many molecular marker techniques to spot the differences. You have decided to do this job with the RAPD technique. You also determined the amount of DNA in the samples you isolated in the spectrophotometer. For Example 1, you measured Sample 1 at 250 ng / microliter. In the RAPD technique, you optimized the total reaction volume for PCR to be 15 microliters. You set this 15 microliters to contain 5 microliters of DNA. You also know that this technique requires 25 ng of DNA to work. How do you dilute the DNA of the 250 ng / microliter sample you have measured into the appropriate amount for the reaction to take place?
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Samples came to your laboratory for analysis. You are asked to identify the differences between these examples. You also learned that in order to catch the difference, I first need to isolate the DNA of the samples. In addition, the possibilities are super ..., chemicals, spectrophotometer, the best centrifuge… so everything is super .. You also isolated the DNA. It's time for the next step. There are many molecular marker techniques to spot the differences. You have decided to do this job with the RAPD technique. You also determined the amount of DNA in the samples you isolated in the spectrophotometer. For Example 1, you measured Sample 1 at 250 ng / microliter. In the RAPD technique, you optimized the total reaction volume for PCR to be 15 microliters. You set this 15 microliters to contain 5 microliters of DNA. You also know that this technique requires 25 ng of DNA to work. How do you dilute the DNA of the 250 ng / microliter sample you have measured into the appropriate amount for the reaction to take place?
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