Question 7The uncoupling agent 2,4-dinitrophenol (DNP) has a pką of about 7.2. Which of thefollowing describes how this molecule works to uncouple the proton gradient?NO2O2N-2,4-Dinitrophenol (DNP)DNP is initially protonated in the intermembrane space, then deprotonatedafter crossing to the matrix, thus dissipating the proton gradient.DNP is initially deprotonated in the intermembrane space, then protonatedafter crossing to the matrix, thus dissipating the proton gradient.O c) None of the answers is correct.DNP is initially deprotonated in the matrix, then protonated after crossing tothe intermembrane space matrix, thus dissipating the proton gradient.DNP is initially protonated in the matrix, then deprotonated after crossing tothe intermembrane space matrix, thus dissipating the proton gradient.

Uncouplers are the compounds that interact with electron transport chain and inner mitochondrial…

Answered: Question 7 The uncoupling agent…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) Figure Q14-18 Essential Cell Biology ale te Garland Science 2010) A NO ATP synthesis since no electron transport occurs. B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created. Click Save and Submit to save and submit. Click Save All Answers to…
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Question 2 Aconitase catalyzes the conversion of Citrate to Isocitrate in the TCA cycle. The standard free energy change (AGO') for this reaction is +6.7 kJ/mol. The observed free energy change (AG) for the reaction in pig heart mitochondria is +0.8 kJ/mol. The ratio of [isocitrate]/[citrate] in these mitrochondria is: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 10 b 1 100 d 0.01 e 3 f 0.33 0.10 0.25 i

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Question 7 The uncoupling agent 2,4-dinitrophenol (DNP) has a pk, of about 7.2. Which of the following describes how this molecule works to uncouple the proton gradient? NO2 O2N- 2,4-Dinitrophenol (DNP)