PIGMENT SPOT Indoxyl Indigo White Indigo Yellow Indigotin

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
icon
Concept explainers
Question
Answer only what is encircled. The explanation is already provided
Heae
asiry
we aDe
2 bydro pbobic
silica gel
plates.
Solverd syslens
solvaol spplem easi
/one of
PAM) and other contaln-
ing
hyd nophobic silica plate can hold haydao phobr coponents
ard so hydrophobie com penuols
less, when wh@ oun tHhe solvent
only very
the compenadts
can move
and
which
move fca above
1 on the
A licatyel plate wll
be morie bydrophi'lic Cas we
cao cusing hydrophobic Silica plate)
Faom the plates o bseaved
ROSI Solvent stystems comporent
fasthest, means
we can
both PAMI and
See
In
D
bas
moved
57
more polaa , follo wed by
Components
C, B
and A.
obsaving the
Indigo yellow has move CA-OH
an ozy gen group capable gf doat29
Now
pigments presepl in indego
groupS
and
alsd
és Cnueleophilic),) hydsophilic gooups
hydrophilic , followad bay ndigo whete Cwith a -01
and 2-NH), then
more
Indoxy) C has
one -oH and
NH groups), and the last , least
indigot in Cunh a-NH)
-GH gooup IS
one
Po lag.
noore polaa than
NH gooup.
Transcribed Image Text:Heae asiry we aDe 2 bydro pbobic silica gel plates. Solverd syslens solvaol spplem easi /one of PAM) and other contaln- ing hyd nophobic silica plate can hold haydao phobr coponents ard so hydrophobie com penuols less, when wh@ oun tHhe solvent only very the compenadts can move and which move fca above 1 on the A licatyel plate wll be morie bydrophi'lic Cas we cao cusing hydrophobic Silica plate) Faom the plates o bseaved ROSI Solvent stystems comporent fasthest, means we can both PAMI and See In D bas moved 57 more polaa , follo wed by Components C, B and A. obsaving the Indigo yellow has move CA-OH an ozy gen group capable gf doat29 Now pigments presepl in indego groupS and alsd és Cnueleophilic),) hydsophilic gooups hydrophilic , followad bay ndigo whete Cwith a -01 and 2-NH), then more Indoxy) C has one -oH and NH groups), and the last , least indigot in Cunh a-NH) -GH gooup IS one Po lag. noore polaa than NH gooup.
This is Thin Layer Chromatography Indigo dye from Marsdenia tinctoria is used
2 hydrophobic silica gel plates are used. They then placed each plate in separate
developing chambers, 2 unknown solvent system. One containing solvent system
PAMI and the other containing solvent system ROSI.
SOLVENT
DISTANCE
SPOTS
SYSTEM
TRAVELLED, cm
solvent
6.4
A
1.4
PAMI
В
1.9
C
4.1
5.3
solvent
6.5
А
0.7
ROSI
В
1.7
В
2.5
В
A
3.0
A
PAMI
ROSI
The indigo mixture from the plant source is known to contain the following pigments.
OH
OH HO
Indoxyl
Indigo White
но
LOH
OH
Indigo Yellow
Indigotin
Based on the structures above, write the letter symbol of the spot corresponding to the
possible-identities of the pigments. Explain your answer.
PIGMENT
Indoxyl
Indigo White
Indigo Yellow
Indigotin
SPOT
Transcribed Image Text:This is Thin Layer Chromatography Indigo dye from Marsdenia tinctoria is used 2 hydrophobic silica gel plates are used. They then placed each plate in separate developing chambers, 2 unknown solvent system. One containing solvent system PAMI and the other containing solvent system ROSI. SOLVENT DISTANCE SPOTS SYSTEM TRAVELLED, cm solvent 6.4 A 1.4 PAMI В 1.9 C 4.1 5.3 solvent 6.5 А 0.7 ROSI В 1.7 В 2.5 В A 3.0 A PAMI ROSI The indigo mixture from the plant source is known to contain the following pigments. OH OH HO Indoxyl Indigo White но LOH OH Indigo Yellow Indigotin Based on the structures above, write the letter symbol of the spot corresponding to the possible-identities of the pigments. Explain your answer. PIGMENT Indoxyl Indigo White Indigo Yellow Indigotin SPOT
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Electronic Effects
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON