Of the steps illustrated how many are unidirectional? Identify the substrate and product of each of these steps. Identify the three enzymes that are likely to be regulated in glycolysis? How did you make this determination. Glycogen synthase is an enzyme that catalyzes the synthesis of glycogen from glucose. Glucose 6 phosphate activates glycogen synthase by allosteric regulation. Illustrate and describe how this allosteric modulation is likely to occur?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
Problem 8RQ
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  1. Of the steps illustrated how many are unidirectional? Identify the substrate and product of each of these steps.
  2. Identify the three enzymes that are likely to be regulated in glycolysis? How did you make this determination.
  3. Glycogen synthase is an enzyme that catalyzes the synthesis of glycogen from glucose. Glucose 6 phosphate activates glycogen synthase by allosteric regulation. Illustrate and describe how this allosteric modulation is likely to occur?
  4. Glycogen phosphorylase, an enzyme that catalyzes the breakdown of glycogen to form glucose, is active when a phosphate group is added. This is an example of enzyme regulation by ____________________________. 

 

 

The following is an illustration of the glycolysis pathway:

1. **Glucose**  
   - Enzyme: Hexokinase  
   - Converts ATP to ADP  
   - Product: Glucose 6-phosphate  

2. **Glucose 6-phosphate**  
   - Enzyme: Phosphoglucose isomerase  
   - Product: Fructose 6-phosphate  

3. **Fructose 6-phosphate**  
   - Enzyme: Phosphofructokinase-1  
   - Converts ATP to ADP  
   - Product: Fructose 1,6-bisphosphate  

4. **Fructose 1,6-bisphosphate**  
   - Enzyme: Aldolase  
   - Splits into:  
     - Glyceraldehyde 3-phosphate  
     - Dihydroxyacetone phosphate  

5. **Dihydroxyacetone phosphate**  
   - Enzyme: Triose phosphate isomerase  
   - Converts to Glyceraldehyde 3-phosphate  

6. **Glyceraldehyde 3-phosphate (2 molecules)**  
   - Enzyme: Glyceraldehyde 3-phosphate dehydrogenase  
   - Converts 2 NAD\(^+\) to 2 NADH  
   - Product: 1,3-Bisphosphoglycerate (2 molecules)  

7. **1,3-Bisphosphoglycerate (2 molecules)**  
   - Enzyme: Phosphoglycerate kinase  
   - Converts 2 ADP to 2 ATP  
   - Product: 3-Phosphoglycerate (2 molecules)  

8. **3-Phosphoglycerate (2 molecules)**  
   - Enzyme: Phosphoglyceromutase  
   - Product: 2-Phosphoglycerate (2 molecules)  

9. **2-Phosphoglycerate (2 molecules)**  
   - Enzyme: Enolase  
   - Releases 2 H\(_2\)O  
   - Product: Phosphoenolpyruvate (2 molecules)  

10. **Phosphoenolpyruvate (2 molecules)**  
    - Enzyme: Pyruvate kinase  
    - Converts 2 ADP to 2 ATP  
    - Product: Pyruvate (2 molecules)
Transcribed Image Text:The following is an illustration of the glycolysis pathway: 1. **Glucose** - Enzyme: Hexokinase - Converts ATP to ADP - Product: Glucose 6-phosphate 2. **Glucose 6-phosphate** - Enzyme: Phosphoglucose isomerase - Product: Fructose 6-phosphate 3. **Fructose 6-phosphate** - Enzyme: Phosphofructokinase-1 - Converts ATP to ADP - Product: Fructose 1,6-bisphosphate 4. **Fructose 1,6-bisphosphate** - Enzyme: Aldolase - Splits into: - Glyceraldehyde 3-phosphate - Dihydroxyacetone phosphate 5. **Dihydroxyacetone phosphate** - Enzyme: Triose phosphate isomerase - Converts to Glyceraldehyde 3-phosphate 6. **Glyceraldehyde 3-phosphate (2 molecules)** - Enzyme: Glyceraldehyde 3-phosphate dehydrogenase - Converts 2 NAD\(^+\) to 2 NADH - Product: 1,3-Bisphosphoglycerate (2 molecules) 7. **1,3-Bisphosphoglycerate (2 molecules)** - Enzyme: Phosphoglycerate kinase - Converts 2 ADP to 2 ATP - Product: 3-Phosphoglycerate (2 molecules) 8. **3-Phosphoglycerate (2 molecules)** - Enzyme: Phosphoglyceromutase - Product: 2-Phosphoglycerate (2 molecules) 9. **2-Phosphoglycerate (2 molecules)** - Enzyme: Enolase - Releases 2 H\(_2\)O - Product: Phosphoenolpyruvate (2 molecules) 10. **Phosphoenolpyruvate (2 molecules)** - Enzyme: Pyruvate kinase - Converts 2 ADP to 2 ATP - Product: Pyruvate (2 molecules)
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  1. Glycogen phosphorylase, an enzyme that catalyzes the breakdown of glycogen to form glucose, is active when a phosphate group is added. This is an example of enzyme regulation by ____________________________. 
**Glycolysis Pathway Overview**

1. **Glucose**
   - Enzyme: Hexokinase
   - Input: ATP
   - Output: ADP
   - Product: Glucose 6-phosphate

2. **Glucose 6-phosphate**
   - Enzyme: Phosphoglucose isomerase
   - Product: Fructose 6-phosphate

3. **Fructose 6-phosphate**
   - Enzyme: Phosphofructokinase-1
   - Input: ATP
   - Output: ADP
   - Product: Fructose 1,6-bisphosphate

4. **Fructose 1,6-bisphosphate**
   - Enzyme: Aldolase
   - Products: Glyceraldehyde 3-phosphate and Dihydroxyacetone phosphate
   
5. **Dihydroxyacetone phosphate**
   - Enzyme: Triose phosphate isomerase
   - Product: Interconverted to Glyceraldehyde 3-phosphate

6. **Glyceraldehyde 3-phosphate**
   - Enzyme: Glyceraldehyde 3-phosphate dehydrogenase
   - Input: 2 NAD\(^+\)
   - Output: 2 NADH + 2 H\(^+\)
   - Product: 1,3-Bisphosphoglycerate (2 molecules)

7. **1,3-Bisphosphoglycerate**
   - Enzyme: Phosphoglycerate kinase
   - Input: 2 ADP
   - Output: 2 ATP
   - Product: 3-Phosphoglycerate (2 molecules)

8. **3-Phosphoglycerate**
   - Enzyme: Phosphoglyceromutase
   - Product: 2-Phosphoglycerate (2 molecules)

9. **2-Phosphoglycerate**
   - Enzyme: Enolase
   - Output: 2 H\(_2\)O
   - Product: Phosphoenolpyruvate (2 molecules)

10. **Phosphoenolpyruvate**
    - Enzyme: Pyruvate kinase
    - Input: 2 ADP
    - Output: 2 ATP
    - Product: Pyruvate (2 molecules)

This diagram illustrates the
Transcribed Image Text:**Glycolysis Pathway Overview** 1. **Glucose** - Enzyme: Hexokinase - Input: ATP - Output: ADP - Product: Glucose 6-phosphate 2. **Glucose 6-phosphate** - Enzyme: Phosphoglucose isomerase - Product: Fructose 6-phosphate 3. **Fructose 6-phosphate** - Enzyme: Phosphofructokinase-1 - Input: ATP - Output: ADP - Product: Fructose 1,6-bisphosphate 4. **Fructose 1,6-bisphosphate** - Enzyme: Aldolase - Products: Glyceraldehyde 3-phosphate and Dihydroxyacetone phosphate 5. **Dihydroxyacetone phosphate** - Enzyme: Triose phosphate isomerase - Product: Interconverted to Glyceraldehyde 3-phosphate 6. **Glyceraldehyde 3-phosphate** - Enzyme: Glyceraldehyde 3-phosphate dehydrogenase - Input: 2 NAD\(^+\) - Output: 2 NADH + 2 H\(^+\) - Product: 1,3-Bisphosphoglycerate (2 molecules) 7. **1,3-Bisphosphoglycerate** - Enzyme: Phosphoglycerate kinase - Input: 2 ADP - Output: 2 ATP - Product: 3-Phosphoglycerate (2 molecules) 8. **3-Phosphoglycerate** - Enzyme: Phosphoglyceromutase - Product: 2-Phosphoglycerate (2 molecules) 9. **2-Phosphoglycerate** - Enzyme: Enolase - Output: 2 H\(_2\)O - Product: Phosphoenolpyruvate (2 molecules) 10. **Phosphoenolpyruvate** - Enzyme: Pyruvate kinase - Input: 2 ADP - Output: 2 ATP - Product: Pyruvate (2 molecules) This diagram illustrates the
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