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Lactobacillus bulgaricus is the main bacterium used in the production of yogurt and requires an acidic environment to grow effectively. To prepare an agar solution of this bacterium, a 100.0 mL 0.100 M buffer solution pH 5.00 must be prepared. The only available reagents in the laboratory are solid benzoic acid (HC6H5COO, MM = 122.12), solid sodium bicarbonate (NaHCO3, MM = 84.01), 1.00 M HCl, and 1.00 M NaOH. Acid dissociation constants: Benzoic acid, HC6H5COO: pKa,1 = 4.19 Carbonic acid, H2CO3: pKa,1 = 6.37, pKa,2 = 10.32 Identify the most appropriate conjugate acid-base pair for the target buffer. Determine the amounts of the acidic and basic species in the buffer (in millimoles).

Lactobacillus bulgaricus is the main bacterium used in the production of yogurt and requires an acidic environment to grow effectively. To prepare an agar solution of this bacterium, a 100.0 mL 0.100 M buffer solution pH 5.00 must be prepared. The only available reagents in the laboratory are solid benzoic acid (HC6H5COO, MM = 122.12), solid sodium bicarbonate (NaHCO3, MM = 84.01), 1.00 M HCl, and 1.00 M NaOH. Acid dissociation constants: Benzoic acid, HC6H5COO: pKa,1 = 4.19 Carbonic acid, H2CO3: pKa,1 = 6.37, pKa,2 = 10.32 Identify the most appropriate conjugate acid-base pair for the target buffer. Determine the amounts of the acidic and basic species in the buffer (in millimoles).

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Lactobacillus bulgaricus is the main bacterium used in the production of yogurt and requires an acidic environment to grow effectively. To prepare an agar solution of this bacterium, a 100.0 mL 0.100 M buffer solution pH 5.00 must be prepared. The only available reagents in the laboratory are solid benzoic acid (HC6H5COO, MM = 122.12), solid sodium bicarbonate (NaHCO3, MM = 84.01), 1.00 M HCl, and 1.00 M NaOH.

Acid dissociation constants:

  • Benzoic acid, HC6H5COO: pKa,1 = 4.19
  • Carbonic acid, H2CO3: pKa,1 = 6.37, pKa,2 = 10.32
  1. Identify the most appropriate conjugate acid-base pair for the target buffer.
  2. Determine the amounts of the acidic and basic species in the buffer (in millimoles).
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