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FLUORESCENCE BINDING a) In the DAPI DNA lab, concentration of DAPI and DNA was determined to carry out analysis. Here is the scenario: 3.50 mL of DAPI at 25 micromolar was placed in the cuvette. It was titrated with a 150 microgram/mL DNA stock. It took 230 microliters of DNA stock to reach saturation. What is the DAPI molarity at saturation in the cuvette? What is the DNA molarity (in terms of base, 1 base = 325 g/mol) at saturation in (i) (ii) cuvette? the

FLUORESCENCE BINDING a) In the DAPI DNA lab, concentration of DAPI and DNA was determined to carry out analysis. Here is the scenario: 3.50 mL of DAPI at 25 micromolar was placed in the cuvette. It was titrated with a 150 microgram/mL DNA stock. It took 230 microliters of DNA stock to reach saturation. What is the DAPI molarity at saturation in the cuvette? What is the DNA molarity (in terms of base, 1 base = 325 g/mol) at saturation in (i) (ii) cuvette? the

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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**Fluorescence Binding** **a)** In the DAPI/DNA lab, concentrations of DAPI and DNA were determined to carry out analysis. Here is the scenario: 3.50 mL of DAPI at 25 micromolar was placed in the cuvette. It was titrated with a 150 microgram/mL DNA stock. It took 230 microliters of DNA stock to reach saturation. **(i)** What is the DAPI molarity at saturation in the cuvette? **(ii)** What is the DNA molarity (in terms of bases, 1 base = 325 g/mol) at saturation in the cuvette? **Calculations from the image:** - **150 μg/mL DNA = 150 x 10⁻⁶ g/mL** The image also includes measurements of: - **230 μL** These calculations are used to determine molarity and saturation levels in the experimental setup.
Transcribed Image Text:**Fluorescence Binding** **a)** In the DAPI/DNA lab, concentrations of DAPI and DNA were determined to carry out analysis. Here is the scenario: 3.50 mL of DAPI at 25 micromolar was placed in the cuvette. It was titrated with a 150 microgram/mL DNA stock. It took 230 microliters of DNA stock to reach saturation. **(i)** What is the DAPI molarity at saturation in the cuvette? **(ii)** What is the DNA molarity (in terms of bases, 1 base = 325 g/mol) at saturation in the cuvette? **Calculations from the image:** - **150 μg/mL DNA = 150 x 10⁻⁶ g/mL** The image also includes measurements of: - **230 μL** These calculations are used to determine molarity and saturation levels in the experimental setup.
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