Complete the table for the unknowns in range: Only complete the math for the table for tubes in range. In range means the absorbance is within the min and max of the standards. Only three unknown tubes in the chart are in range. Complete the math below and fill in the table for those three. Write Out of Range in the table for the two that are out of range.

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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5. GRAPH: Graphing the data from the Carbohydrate and Protein assays is the same process. This is
practice to ensure you know how to use Excel to graph, obtain a best fit line, and equation. Do this in
Excel and write the obtained equation (y-mx+b) somewhere near the table.
Assay Tube
1 (PBS)
2
3
4
5
6
7
Unknown sample
Original Stock
(100%)
Unknown
1:10 dilution
Unknown
1:100 dilution
Unknown
1:1000 dilution
Unknown
1:10000 dilution
Standard
(mg/ml)
0
0.125
0.250
0.500
0.750
1.00
1.500
Absorbance
at 595 nm:
(A)
0
0.100 A
0.202 A
0.428 A
0.477 A
0.612 A
0.868 A
1.971 A
1.814 A
0.572 A
0.088 A
0.206 A
6. Complete the table for the unknowns in range: Only complete the math for the table for tubes in
range. In range means the absorbance is within the min and max of the standards. Only three unknown
tubes in the chart are in range. Complete the math below and fill in the table for those three. Write Out
of Range in the table for the two that are out of range.
Transcribed Image Text:5. GRAPH: Graphing the data from the Carbohydrate and Protein assays is the same process. This is practice to ensure you know how to use Excel to graph, obtain a best fit line, and equation. Do this in Excel and write the obtained equation (y-mx+b) somewhere near the table. Assay Tube 1 (PBS) 2 3 4 5 6 7 Unknown sample Original Stock (100%) Unknown 1:10 dilution Unknown 1:100 dilution Unknown 1:1000 dilution Unknown 1:10000 dilution Standard (mg/ml) 0 0.125 0.250 0.500 0.750 1.00 1.500 Absorbance at 595 nm: (A) 0 0.100 A 0.202 A 0.428 A 0.477 A 0.612 A 0.868 A 1.971 A 1.814 A 0.572 A 0.088 A 0.206 A 6. Complete the table for the unknowns in range: Only complete the math for the table for tubes in range. In range means the absorbance is within the min and max of the standards. Only three unknown tubes in the chart are in range. Complete the math below and fill in the table for those three. Write Out of Range in the table for the two that are out of range.
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