Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor)| If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
icon
Concept explainers
Question
Calculate the following DNA concentrations and determine the purity of the samples:
DNA
Dilution
Dilution
A260
Concentration A260/280
Pure
Explain
Sample
factor
DNA?
1
1 ul in a total
10/1 =
1.98
1.56
volume of
10
10ul
2
0.5 ul in a
20
2.5
1.82
total volume
of 10ul
3
5 ul in a total
0.86
1.95
volume of 25
ul
Show your calculations (for samples 1-3 above) below:
DNA conc = (A260 reading) x (absorbance factor) x (dilution factor)
If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield
(concentration)? Show your calculations below:
Transcribed Image Text:Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor) If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:
Expert Solution
steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Nucleic Acids
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY