a. Outline and describe the reaction scheme of the production of glucose from maltodextrin by using glucoamylase.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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a. Outline and describe the reaction scheme of the production of glucose from maltodextrin
by using glucoamylase.
Transcribed Image Text:a. Outline and describe the reaction scheme of the production of glucose from maltodextrin by using glucoamylase.
Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus
niger at different temperatures in the production of glucose from maltodextrin. The rates of
reaction measured are using an enzymes concentration of 0.003 μmolml-¹.
Table 1: Glucoamylase Activity at different temperatures
Maltodextrin Concentration
(μmol/L)
0.5
1
1.5
2.2
5.5
11
Initial Reaction Velocity
(μmol/L.s)
T = 40°C
2.1
3.5
4.9
5.6
589
8.3
9.3
T = 60°C
5.2
9.7
13
16
23
31
Transcribed Image Text:Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at different temperatures in the production of glucose from maltodextrin. The rates of reaction measured are using an enzymes concentration of 0.003 μmolml-¹. Table 1: Glucoamylase Activity at different temperatures Maltodextrin Concentration (μmol/L) 0.5 1 1.5 2.2 5.5 11 Initial Reaction Velocity (μmol/L.s) T = 40°C 2.1 3.5 4.9 5.6 589 8.3 9.3 T = 60°C 5.2 9.7 13 16 23 31
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