A column 3.00 cm in diameter and 32.6 cm long gives adequate resolution of a 72.4-mg mixture of unknowns, initially dissolved in 0.500 mL. (a) If you wish to scale down to 10.0 mg of the same mixture with minimum use of chromatographic stationary phase and solvent, what length and diameter column would you use? (b) In what volume would you dissolve the sample? (c) If the flow rate in the large column is 1.85 mL/min, what should be the flow rate in the small column?

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Unit Conversion

Standard units are also called SI units. SI units are used for measuring a physical quantity satisfying certain conditions. There are two types of units: fundamental units and derived units. Fundamental units are physical quantities that are independent, that is, they do not depend on other quantities like mass, length, or time. There are plenty of quantities in our lives which need to be measured and compared. Measuring is the comparison of a particular physical quantity with its reference value. A unit is a measure of a fixed physical quantity. A physical quantity is the product of a unit and the numerical value we need to compare with.