75 6.5× 10° 2.2 × 10° 4.1 × 105 5.1 × 105 7.9 × 105 7.7 × 105 6.0 × 105 5.5 × 105 3.5 × 105 1.7 × 105 1.3 × 105 1.1 × 106 1.0× 106 9.7 × 10* 1.6 x 105 2.5 x 105 100 125 9.7× 105 7.8× 105 7.0× 105 7.0× 105 6.5× 105 4.7 × 105 4.2× 105 150 4.1 × 105 5.9 × 105 5.4 x 105 4.4 × 105 2.6 × 105 1.2 × 105 8.8 × 10* 175 200 225 250 275 300 (a) Does medium osmolarity affect whether the hybridoma cells undergo a lag phase? (b) How does medium osmolarity affect the maximum specific growth rate of the cells? (c) How does medium osmolarity affect the maximum cell concentration achieved and the tim

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
Viable Cell Concentration (cells ml¬1)
Osmolarity =
Time (h) 290 mOsm 1¯1 380 mOsm 1¬1
Osmolarity =
Osmolarity =
435 mOsm 1¬1
4.2× 10ª
9.8 × 10ª
2.2 × 105
6.5× 105
1.1× 106
1.0× 106
9.7× 105
7.8× 105
7.0× 105
7.0× 105
6.5× 105
4.7× 105
4.2x 105
4.2 × 10ª
6.3 × 10*
1.2 x 105
2.2 × 105
4.1 × 105
5.1 × 105
7.9 × 105
7.7 × 105
6.0 × 105
5.5 x 105
3.5 × 105
1.7 × 105
1.3 × 105
< 10*
4.8 × 10ª
7.0 × 104
4.2 x 1
25
50
75
9.7 × 10*
1.6 x 105
2.5 x 105
100
125
150
4.1 × 105
5.9 × 105
5.4 × 105
4.4 × 105
2.6 × 105
1.2 × 105
8.8 × 10*
175
200
225
250
275
300
(a) Does medium osmolarity affect whether the hybridoma cells undergo a lag phase?
(b) How does medium osmolarity affect the maximum specific growth rate of the cells?
(c) How does medium osmolarity affect the maximum cell concentration achieved and the time
taken to reach maximum cell density?
(d) What effect does medium osmolarity have on the specific death rate of the cells during the
culture death phase? Medium sterilization.
Transcribed Image Text:Viable Cell Concentration (cells ml¬1) Osmolarity = Time (h) 290 mOsm 1¯1 380 mOsm 1¬1 Osmolarity = Osmolarity = 435 mOsm 1¬1 4.2× 10ª 9.8 × 10ª 2.2 × 105 6.5× 105 1.1× 106 1.0× 106 9.7× 105 7.8× 105 7.0× 105 7.0× 105 6.5× 105 4.7× 105 4.2x 105 4.2 × 10ª 6.3 × 10* 1.2 x 105 2.2 × 105 4.1 × 105 5.1 × 105 7.9 × 105 7.7 × 105 6.0 × 105 5.5 x 105 3.5 × 105 1.7 × 105 1.3 × 105 < 10* 4.8 × 10ª 7.0 × 104 4.2 x 1 25 50 75 9.7 × 10* 1.6 x 105 2.5 x 105 100 125 150 4.1 × 105 5.9 × 105 5.4 × 105 4.4 × 105 2.6 × 105 1.2 × 105 8.8 × 10* 175 200 225 250 275 300 (a) Does medium osmolarity affect whether the hybridoma cells undergo a lag phase? (b) How does medium osmolarity affect the maximum specific growth rate of the cells? (c) How does medium osmolarity affect the maximum cell concentration achieved and the time taken to reach maximum cell density? (d) What effect does medium osmolarity have on the specific death rate of the cells during the culture death phase? Medium sterilization.
2) Effect of medium osmolarity on growth and death of hybridoma cells. Medium
osmolarity is an important variable in the design of serum-free media for mammalian cell
culture. A murine hybridoma cell line synthesising IgG1 antibody is grown in culture
media adjusted to three different osmolarities by addition of NaCl or sucrose. Growth and
viability of the cells in batch culture are monitored over a period of 300 hours. Results for
viable cell concentration as a function of culture time are as follows.
Transcribed Image Text:2) Effect of medium osmolarity on growth and death of hybridoma cells. Medium osmolarity is an important variable in the design of serum-free media for mammalian cell culture. A murine hybridoma cell line synthesising IgG1 antibody is grown in culture media adjusted to three different osmolarities by addition of NaCl or sucrose. Growth and viability of the cells in batch culture are monitored over a period of 300 hours. Results for viable cell concentration as a function of culture time are as follows.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Organ system
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education