1. Pro A wild-type strain of bacteria produces a protein with the amino acid proline (Pro) at one site. Treatment with nitrous acid causes a C to change to a U. Two different mutants are produced after nitrous acid treatment of the bacteria. One has a serine (Ser) in place of the proline, an the other has a leucine (Leu). Further treatment of the two mutants with nitrous acid produces new mutant strains, both with phenylalanine (Phe) at the site. Treatment of the phenylalanine carrying mutants produces no change. U Ser Leu Phe →Phe UUC UUUA UUG с UUU Phe Leu G CUU CUC CUA CUG AUU- AUC lle A AUA Leu AUG-Met GUU GUC GUA GUG Val с UCU UCC UCA UCG CCU CCC CCA CCG- ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala A UAU Tyr Cys UAC UAA Stop UGA-Stop UAG UGG Trp CAU His CAC CAA CAG GAU GAC GAA GAG Gin AAC Asn AAA AAG Lys Asp UGU UGC Glu CGU CGC CGA CGG AGU AGC AGA AGG GGU GGC GGA GGG G b. Explain why further treatment of the phenylalanine mutants with nitrous acid produces no change. Arg Ser Arg Gly a. Use appropriate codons to show how these mutations occurred. Assume only a single nucleotide change occurs with each treatment of nitrous acid.
1. Pro A wild-type strain of bacteria produces a protein with the amino acid proline (Pro) at one site. Treatment with nitrous acid causes a C to change to a U. Two different mutants are produced after nitrous acid treatment of the bacteria. One has a serine (Ser) in place of the proline, an the other has a leucine (Leu). Further treatment of the two mutants with nitrous acid produces new mutant strains, both with phenylalanine (Phe) at the site. Treatment of the phenylalanine carrying mutants produces no change. U Ser Leu Phe →Phe UUC UUUA UUG с UUU Phe Leu G CUU CUC CUA CUG AUU- AUC lle A AUA Leu AUG-Met GUU GUC GUA GUG Val с UCU UCC UCA UCG CCU CCC CCA CCG- ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala A UAU Tyr Cys UAC UAA Stop UGA-Stop UAG UGG Trp CAU His CAC CAA CAG GAU GAC GAA GAG Gin AAC Asn AAA AAG Lys Asp UGU UGC Glu CGU CGC CGA CGG AGU AGC AGA AGG GGU GGC GGA GGG G b. Explain why further treatment of the phenylalanine mutants with nitrous acid produces no change. Arg Ser Arg Gly a. Use appropriate codons to show how these mutations occurred. Assume only a single nucleotide change occurs with each treatment of nitrous acid.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Pro
A wild-type strain of bacteria produces a protein with the amino acid proline (Pro) at one site. Treatment with
nitrous acid causes a C to change to a U. Two different mutants are produced after nitrous acid treatment of
the bacteria. One has a serine (Ser) in place of the proline, an the other has a leucine (Leu). Further treatment
of the two mutants with nitrous acid produces new mutant strains, both with phenylalanine (Phe) at the site.
Treatment of the phenylalanine carrying mutants produces no change. U
Ser
Leu
Phe
→Phe
U
с
A
บบบ
UUC
UUA LOU
UUG
CUU
CUC
CUA
CUG-
Phe
GUU
GUC
G GUA
GUG
Leu
AUU
AUC lle
AUA
AUG-Met
Val
с
UCU
UCC
UCA
UCG
CCU
CCC
CCA
CCG
ACU
ACC
ACA
ACG
GCU
GCC
GCA
GCG
Ser
Pro
Thr
Ala
A
UAU
Tyr
Cys
UAC
UAA Stop UGA-Stop
UAG
UGG-Trp
CAU His
CAC
CGU
CGC
CAA Gin CGA
CAG
CGG
AAU Asn
AAC
AAA
AAG Lys
UGU
UGC
GAU
GAC
GAA
GAG
Asp
Glu
AGU
AGC
AGA
AGG
GGU
GGC
GGA
GGG
G
b. Explain why further treatment of the phenylalanine mutants with nitrous acid produces no change.
Arg
Ser
Arg
Gly
a. Use appropriate codons to show how these mutations occurred. Assume only a single nucleotide change
occurs with each treatment of nitrous acid.
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