lab 1
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Texas A&M University *
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111
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Mathematics
Date
Apr 3, 2024
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docx
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5
Uploaded by oliviabogard1
To help illustrate the i e the importance of i ; som ; : following case studies in class with youre I:l: ii;;itsri;tifrety items, you will work through the Case studies: Case Study 1 Observe the image shown and answer the prompts below. 1. List 5 good safety practices seen in the picture. Briefly describe why these are important. o 60@3\&5 - protedhs eyes s Slows - protecks hands /st 5 Q(’TNS - F/OTU)FS 0()0'(@»«,3/ bfiflj o ARd shoes — preas feet o S e T N-tuw Bre E . 2. List 5 unsafe activities seen in the picture. How are they unsafe? D AP A ¢« NOY V\)‘(fl”flj fiNS i M/O’k P-fi) T Zzu bfu/%g/ Wore MISAE Catta «y Pt Unenigicas — | nd { — (DU Lavsc buak vesn Its ¢ Lood ln lab ~ Un Contaminated ~—Asate 7 zet &/C&Vbcq/-@&(MSMma d respond to the prompt below. Read the text provided an riment do you see? How could this be 1. What hazards to your lab partner and the expe prevented? Aoy Wi N put o @ Ao plic | P e B twn e prernted by é
Case Study 3 Read the text provided and respond to the prompts below. 1. What should you do now? IO Wash Liges n Sl 2. How could this have been prevented? e U ool ¢qun ower actod? puy Otmbim {0 Ao ped't amonAt o Case Study 4 Read the text provided and respond to the prompts below. 1. What issues did you notice in this scenario? e R4S don - Ol opin fork she S uld these unfortunate incidents have been prevented? (/kawklv@ skl Puw p lagies ¢ a ny Pl Closet foeet shoss or sk Por oo s 2. How co
into the tube, gently pipet the solution up and down to mix. Cap the tube, invert and flick the tube to mix further. 4. Repeatstep 3, by adding tubes, respectively. Mix. 5. To create the VA tube, add 100 uL solution using the P-20. Mix . To create the O1 tube, add 100 uL of yellow dye and 100 ulL blue dye to the Y1 and B1 of the R1 solution using the P-200, and 10 uL of the B1 4100 uL of Y1 solution and 20 uL of the R1 solution. Mix. 6 7. To create the G1 tube, add 100 uL of Y1 solution and 20 uL of the B1 solution. Mix. 8. Obtain6-1.5mL microcentrifuge tubes and label them: R2, 02, Y2, G2, B2, and V2. 9 1 . Using the P-1000, put 900 uL of water in each of the tubes labeled in step 8. 0. Using your P-200, transfer 100 uL of R1, 01, Y1, G1, B1 and V1 into the R2, 02, Y2, G2, B2. and V2 tubes, respectively. Mix. 11. Obtain 6 - 1.5 mL microcentrifuge tubes and label them R3, 03, Y3. G3, B3, and V3. 12. Using the P-1000, put 9 00 uL of water in each of the tubes labeled in step 12. 13. Using your P-200, trans R3,03.Y3.G3. fer 100 ulL of R2, 02, Y2, G2, B2 and V2 into the B3, and V3 tubes, respectively. A. Observe your three series of tubes. What do you notice about the intensity of the color? A oW guts gty o e B. After creating the series 1 tubes, you diluted them to 1:10 (100 uL in 900 u = 1000 ul) to create the seri ( L ies 2 tubes. You then diluted the series 2 tu series 3. If the series 1 tu R e bes had an initial concentration of 1 unit/m the concentration of the series 3 tubes? ve it eg ege Thinking about the example you just worked i TR e, through, use the below image to answer the
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Stock A B (4 D E Final a. What is the dilution between the stock solution and Tube C? b. What is the dilution factor for the FINAL tube? L [Rec c. What is the dilution between Tubes A and B? b
—— 1. How many uL of your stock solution (2mg/mL) would you need to add to get a final concentration of 500ug/mL in a volume of 1mL? Cil/y "Ca Vq Ofl X\/\—/ 'g)( \ Qf(/“:/ ,"isw\L —1 nguL 2. You are making some TAE buffer for the lab and you mix 100mL of your Stock (50mg/mL) with water to obtain a final volume of 4L. What is the concentration of the new TAE buffer? |00 X S0 iyt Evon -t C = 1250 mglml 3. In the lab you have a stock solution of buffer (10mg/mL). You transfer 5ul to a new tube, but have forgotten what your final volume should be. You remember that you need to make a diluted solution that is 50ug/mL in concentration. What should be the final volume (in mL) of your diluted solution? 10 ¥ .005 - SOAY, MO SHEREBX \/; = ohilhaE 4. You are given a protocol that tells you to add 10uL of your stock solution to a tube. It then tells you to use water to bring it up to a final volume of 500uL. The protocol says this will give you a working solution that is 75ug/mL in concentration. What is the concentration (in mg/mL) of your stock solution given this information? Vi= .DlmL Vy = sml . C‘ :'DVISMQ L ,O‘/lq)(,b] - CQ_X A EllER e = o &