PhysioEx Exercise 1 Activity 5

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Washington State University *

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Chemistry

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Jan 9, 2024

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1/28/23, 11:48 PM PhysioEx Exercise 1 Activity 5 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/ 1/3 PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Fatoumata Darboe Date: 28 January 2023 Session ID: session-0383cb7d-99c7-b5c1-92ae-c6fa84f4831c Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Questions Stop & Think Questions The movement of sodium and potassium maintained by the Na -K pump You correctly answered: requires energy and is against a concentration gradient. 1 + + The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 2 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 3 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble. 4 Predict Question 1: What do you think will result from these experimental conditions? Your answer: Na will be partially transported. 1 + Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion. 2 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted. 1 h h l b f h l h d l
1/28/23, 11:48 PM PhysioEx Exercise 1 Activity 5 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/ 2/3 Experiment Data Run Number Solute ATP (mM) Start Conc. L (mM) Start Conc. R (mM) Pumps Carriers Rate (mM/sec) 1 Na Cl 1 9.000 0.000 500 --- 0.0188 1 K Cl 1 0.000 6.000 500 --- 0.0125 1 Glucose --- 0.000 0.000 --- 0 0.0000 2 Na Cl 3 9.000 0.000 500 --- 0.0025 2 K Cl 3 0.000 6.000 500 --- 0.0017 2 Glucose --- 0.000 0.000 --- 0 0.0000 3 Na Cl 3 9.000 0.000 500 --- 0.0000 3 K Cl 3 0.000 0.000 500 --- 0.0000 3 Glucose --- 0.000 0.000 --- 0 0.0000 4 Na Cl 3 9.000 0.000 800 --- 0.0042 4 K Cl 3 0.000 6.000 800 --- 0.0028 4 Glucose --- 0.000 0.000 --- 0 0.0000 5 Na Cl 3 9.000 0.000 800 --- 0.0042 5 K Cl 3 0.000 6.000 800 --- 0.0028 5 Glucose --- 0.000 10.000 --- 400 0.0028# Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Why was the equilibrium for the solutes reached earlier? Your answer: The solute concentrations were higher. Correct answer: There were more pumps for transport. 2 + - + - + - + - + - + - + - + - + - + - What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane? You correctly answered: More ions were transported. 1 At what concentration of ATP were the sodium and potassium maximally transported? You correctly answered: 3 mM ATP. 2 What was the effect of adding more Na -K pumps to the simulated cell? You correctly answered: Transport of the ions was faster. 3 + + Describe the effect of adding glucose carriers to the sodium and potassium transport. 4
1/28/23, 11:48 PM PhysioEx Exercise 1 Activity 5 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/ 3/3 Review Sheet Results You correctly answered: There was no change in the transport rate because glucose is transported independently. Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: The ratio is 3/2 and it works out because you need 3 sodium ions taken out for every 2 potassium ions moved in. 1 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: I thought some Na would be transported but according to the results, it doesn't seem like any was transported. They probably weren't able to be transported because there was no sodium-potassium pumps. 2 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: I thought it would have no effect because if it is a glucose carrier it would only be able to transport glucose and the results reflect that. 3 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: Glucose is being transported by facilitated diffusion because it moves down the concentration gradient. 4
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