PhysioEx Exercise 1 Activity 5
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PhysioEx Exercise 1 Activity 5
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PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability
Activity 5: Simulating Active Transport
Name: Fatoumata Darboe
Date: 28 January 2023
Session ID: session-0383cb7d-99c7-b5c1-92ae-c6fa84f4831c
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Experiment Results
Predict Questions
Stop & Think Questions
The movement of sodium and potassium maintained by the Na
-K
pump
You correctly answered:
requires energy and is against a concentration gradient.
1
+
+
The sodium-potassium pump is classified as a(n)
You correctly answered:
antiporter.
2
The sodium-potassium pump moves _____ sodium ions and _____ potassium ions
simultaneously.
You correctly answered:
3, 2.
3
Solutes that require active transport for movement might be too large to pass or might be
You correctly answered:
lipid insoluble.
4
Predict Question 1: What do you think will result from these experimental conditions?
Your answer:
Na
will be partially transported.
1
+
Predict Question 2: Do you think the addition of glucose carriers will affect the transport of
sodium or potassium?
Your answer:
No, it will not affect the transport of either ion.
2
Why did the sodium transport stop before the transport was completed?
You correctly answered:
The ATP was depleted.
1
h
h
l b
f
h
l
h d
l
1/28/23, 11:48 PM
PhysioEx Exercise 1 Activity 5
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Experiment Data
Run
Number
Solute
ATP (mM)
Start
Conc. L
(mM)
Start
Conc. R
(mM)
Pumps
Carriers
Rate
(mM/sec)
1
Na
Cl
1
9.000
0.000
500
---
0.0188
1
K
Cl
1
0.000
6.000
500
---
0.0125
1
Glucose
---
0.000
0.000
---
0
0.0000
2
Na
Cl
3
9.000
0.000
500
---
0.0025
2
K
Cl
3
0.000
6.000
500
---
0.0017
2
Glucose
---
0.000
0.000
---
0
0.0000
3
Na
Cl
3
9.000
0.000
500
---
0.0000
3
K
Cl
3
0.000
0.000
500
---
0.0000
3
Glucose
---
0.000
0.000
---
0
0.0000
4
Na
Cl
3
9.000
0.000
800
---
0.0042
4
K
Cl
3
0.000
6.000
800
---
0.0028
4
Glucose
---
0.000
0.000
---
0
0.0000
5
Na
Cl
3
9.000
0.000
800
---
0.0042
5
K
Cl
3
0.000
6.000
800
---
0.0028
5
Glucose
---
0.000
10.000
---
400
0.0028#
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Why was the equilibrium for the solutes reached earlier?
Your answer:
The solute concentrations were higher.
Correct answer:
There were more pumps for transport.
2
+
-
+
-
+
-
+
-
+
-
+
-
+
-
+
-
+
-
+
-
What happened when you increased the amount of ATP dispensed with the same
concentration of sodium and potassium on either side of the membrane?
You correctly answered:
More ions were transported.
1
At what concentration of ATP were the sodium and potassium maximally transported?
You correctly answered:
3 mM ATP.
2
What was the effect of adding more Na
-K
pumps to the simulated cell?
You correctly answered:
Transport of the ions was faster.
3
+
+
Describe the effect of adding glucose carriers to the sodium and potassium transport.
4
1/28/23, 11:48 PM
PhysioEx Exercise 1 Activity 5
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Review Sheet Results
You correctly answered:
There was no change in the transport rate because glucose is
transported independently.
Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium
chloride outside the cell, instead of other concentration ratios.
Your answer:
The ratio is 3/2 and it works out because you need 3 sodium ions taken out for every 2
potassium ions moved in.
1
Explain why there was no sodium transport even though ATP was present. How well did the
results compare with your prediction?
Your answer:
I thought some Na would be transported but according to the results, it doesn't seem like
any was transported. They probably weren't able to be transported because there was no
sodium-potassium pumps.
2
Explain why the addition of glucose carriers had no effect on sodium or potassium transport.
How well did the results compare with your prediction?
Your answer:
I thought it would have no effect because if it is a glucose carrier it would only be able to
transport glucose and the results reflect that.
3
Do you think glucose is being actively transported or transported by facilitated diffusion in
this experiment? Explain your answer.
Your answer:
Glucose is being transported by facilitated diffusion because it moves down the
concentration gradient.
4
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