Lab2_assign_worksheet

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Harvard University *

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2071

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Biology

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Jan 9, 2024

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Name: Lab section : BIOL 2071 Introductory Microbiology & Techniques Worksheet for Lab #2 /10 This worksheet is due at the end of Lab #2. Pre-Lab Questions Complete these questions prior to coming into lab. Point-form answers are acceptable. 1. Differentiate between simple and differential stains. Provide an example of each. /2 2. What is the major structural difference between Gram positive (+) and negative (–) bacteria. How do they appear when Gram stained? /1 3. In Gram staining, what is the purpose of the counterstain? /1 4. What should be done differently when preparing smears from solid media versus broth cultures? /1 GA/TA initial: COMPLETE THE FOLLOWING BASED ON LAB #1 RESULTS Part 1: Aseptic technique Observe your results from last week’s aseptic technique and in your lab notebook, describe the appearance of your inoculated slants, broths and Tsoy plate. Use colour, morphology and overall growth as descriptors. If you obtained unexpected results, record possible sources of error. Part 2: Isolation of yeast using the streak plate technique BIOL 2071: Introductory Microbiology & Techniques, Fall 2023 – Worksheet for Lab #2
Compare the appearance of the colonies on the mixed plate. Describe the two different types of colonies you see, naming them 1 and 2 and record this information in your lab notebook . COMPLETE THE FOLLOWING QUESTIONS BASED ON IN-LAB EXERCISES 5. Complete the tables below based on the appearance of the bacteria under the microscope, preferably using the 100X objective. Include descriptions of shape, cell organization, and colour. For Gram stains, be sure to indicate whether the bacteria are Gram (+) or (-). (a) /2 Simple stains S. epidermidis B. subtilis Appearance under the microscope Shape Organization Colour (b) /3 Gram stains S. epidermidis E. coli B. subtilis Appearance under the microscope Shape Organization Colour Gram reaction BIOL 2071: Introductory Microbiology & Techniques, Fall 2023 – Worksheet for Lab #2
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