Lab 5_ Histology of Epithelial Tissue Lab Exercises

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Mesa Community College *

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201

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Biology

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Apr 3, 2024

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Lab 5: Histology of Epithelial Tissue Lab Exercises Student Name: ____________________________ General Directions ● Answer the questions for each tissue type. Your “Background” document and the lab videos on Canvas will help you to answer these questions. ● Examine each type of tissue listed below. Use the linked lab videos on Canvas and the “Epithelial Tissue Slides” Google Photo album to help you identify tissues and structures within the tissues as you make your drawings. ● Draw each tissue to better identify and remember it. When drawing the different tissue types focus on: the shape of the cells and how you will recognize this tissue when you see it on the practical exam. Note that on the practical exam you will see new images that will be a different photo of the same tissue . Record each tissue type at the suggested total magnification by: a. Pausing the video and drawing a picture of each specific epithelial tissue on a piece of paper. b. Label each of your drawings/pictures as directed below. c. Every drawing should include the total magnification of the specimen (high power is 400x total magnification). Epithelial Tissue Questions 1. What is the lumen? In which epithelial tissues would you find a lumen? Lumens are the clear areas of the epithelium or the tubule insides. These can be found in the simple cuboidal epithelium which found in the outer layer of kidney tubules, surface lens of the eye, follicles of the thyroid gland and other glands that use secretion. 2. What is the function of the goblet cells? In which epithelial tissues would you find a goblet cell? Goblet cells are found in the simple columnar epithelium and are used to secrete mucus. This helps lubricate and protect the digestive track from being destroyed. 3. What is the function of the germinal layer? In which epithelial tissues would you find a germinal layer? (Hint: This layer is found in epithelium with more than one layer.) The germinal layer is found in the nonkeratinized stratified squamous epithelium and its function is to be the bottom layer of a stratified cell that the newer cells rest on as the older cells get pushed toward the surface to eventually fall off. 4. What is keratin? What is the main function of the tissue that contains keratin (keratinized stratified squamous epithelium)? Keratin is the protein accumulated by mature cells on the epidermis of the skin. It is the hardened waste of the dead squamous cells. The keratinized layers function is to protect from bacterial invasion and abrasion. BIO201 @ MCC Lab 5: Epithelial Tissue, Page 1

5. List two locations in the human body that each specific type of epithelial tissue may be found. Be specific since many organs are made up of more than one tissue type. Epithelial Tissue Type Two Locations in Human Body Simple squamous epithelium 1. Lining of the air sacs of the lungs 2. Inner surface of eardrum and the canal system of inner ear Simple cuboidal epithelium 1. Microscopic kidney tubules 2. Surface of ovary and lens of the eye Simple columnar epithelium 1. Lining of the gallbladder 2. Alimentary canal from stomach to anus Ciliated pseudostratified columnar epithelium 1. Respiratory track of nasal cavity through bronchi of lungs 2. Eustachian tubes Nonkeratinized stratified squamous epithelium 1. Lining of mouth 2.Lining of esophagus Keratinized stratified squamous epithelium 1. epidermis of the skin 2. n/a (only found in one location) Transitional epithelium 1. Lining of uterus 2. Lining of urinary bladder Drawing Epithelial Tissues Directions ● Using the lab videos, draw each tissue to better identify and remember it. You may use any paper and pencil you have handy, but a blank paper (no lines) and pencil work well. Feel free to add color if you would like. ● Be sure to add the labels as required below, using the online photos to help you label the required structures. Drawing what you see encourages you to look at the details of the specimen and helps to to learn to identify the cells and cellular structures. ● When you complete your drawings, take a photo or scan and upload to Canvas under the “Lab 5: Histology of Epithelial Tissue Lab Exercise Upload” assignment. Simple Squamous Epithelium 1. Draw the cross section view of simple squamous epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, nucleus and free surface. Make sure you also view the top view of simple squamous epithelium (no drawing required). BIO201 @ MCC Lab 5: Epithelial Tissue, Page 2

Simple Cuboidal Epithelium 2. Draw the cross section view of simple cuboidal epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, lumen, basement membrane, nucleus, and free surface. Simple Columnar Epithelium 3. Draw the cross section view of simple columnar epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, nucleus, lumen, and goblet cell. Ciliated Pseudostratified Columnar Epithelium 4. Draw the cross section of ciliated pseudostratified columnar epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, cilia, goblet cell, basement membrane, and nucleus. Nonkeratinized Stratified Squamous Epithelium 5. Draw the cross section view of nonkeratinized stratified squamous epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, lumen, free surface, germinal layer, and basement membrane. Keratinized Stratified Squamous Epithelium 6. Draw the cross section view of keratinized stratified squamous epithelial tissue at 100x total magnification (low power). Identify and label the tissue type, total magnification, germinal layer, and keratinized layer. You will look at this tissue in more detail in Lab 7: Skin. Transitional Epithelium 7. Draw the cross section view of transitional epithelial tissue at 400x total magnification (high power). Identify and label the tissue type, total magnification, lumen, free surface, germinal layer and basement membrane. BIO201 @ MCC Lab 5: Epithelial Tissue, Page 3

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Drawings of Epithelial Tissues (see above for directions) Specific Tissue Type (full name): Specific Tissue Type (full name): Total magnification: Total magnification: Picture Picture Specific Tissue Type (full name): Specific Tissue Type (full name): Total magnification: Total magnification: Picture Picture Specific Tissue Type (full name): Specific Tissue Type (full name): BIO201 @ MCC Lab 5: Epithelial Tissue, Page 4

Total magnification: Total magnification: Picture Picture Specific Tissue Type (full name): Total magnification: BIO201 @ MCC Lab 5: Epithelial Tissue, Page 5

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