Pipetting Postlab Template

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James Madison University *

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140

Subject

Biology

Date

Apr 3, 2024

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pdf

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2

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BIO140: DNA Barcoding Pipetting and DNA Extraction Postlab Name: ___________________________ Please complete this postlab individually, and submit it electronically via Canvas by the start of the next lab. 8 points total. 1. Copy your pipetting results from exercise A3 into the table below. (1 pt) Volume pipetted Mass (g) 1000 μL 500 μL 250 μL 2. Using your cell phone, take a picture of your half circle from exercise 2 in part A3 and insert in the space below. Make sure you write your name on your filter paper before taking a picture. (1 pt) 3. What pipetting technique should you use to ensure that you properly dispense 2 μL of a DNA extract into a PCR reaction tube? (1 pt) 4. Which pipettor should you use to accurately pipet 20 μL? The 2-20 μL pipettor, or the 20-200 μL pipettor? Why? (1 pt)
5. On the diagrams below, fill in circles to demonstrate two different ways to balance three microcentrifuge tubes in a centrifuge rotor. You may use a balance tube in one of your diagrams. (1 pt) 6. Why is it important to make sure the lid is securely attached to a centrifuge rotor? (1 pt) 7. What are two methods for preventing contamination in the laboratory? Why is preventing DNA contamination in the laboratory so important? (1 pt) 8. What are two differences between the plant/fungal DNA extraction protocol and the invertebrate/fish DNA extraction protocol? Please be specific! (1 point)
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