Experiment 2 - Lab Report
Characterization of Phosphatase Enzyme
Inserting your answers after each question/space. Please denote your answers by using
bold
,
italic
s, or
colored
font. Do NOT renumber/reorganize the questions.
“Showing your work/calculations” can be achieved by typing it out, pasting in a clear
image of your handwritten work, or using handwritten annotation on the document (if
you have the technology to do so).
Insert graphs (handwritten on graph paper or electronically constructed with
horizontal/vertical gridlines) as a clear image with appropriate titles, labeled/scaled
axes, and standard deviation bars when appropriate.
1.
(6pts) Construct two separate bar graphs depicting your results for the effect of pH and
temperature on phosphatase activity.
All figures, graphs, and tables must include appropriate
titles, legends, labeled/scaled axes, and standard deviation bars.
Do not
include a figure caption.
* If you experience problems adding custom error bars with Excel/Google sheet graphs, hand-
drawn error bars are acceptable.
2.
(2pts) Show all
the calculations for the pH 5 data point that was plotted on your pH graph.
3.
(3pts) Based upon your data,
a)
What was the temperature optimum of the phosphatase assay?
b)
What was the pH optimum of the phosphatase assay?
c)
How would you
classify
the phosphatase activity found in your extract?
4.
(4pts) Write an abstract for this experiment.
An abstract is a brief paragraph (three to five
sentences) that communicates the purpose, the experiment conducted, and the specific
conclusions of the experiment.
Abstract should be concise without going into details about the
protocol. Each sentence should convey required, non-redundant information.
5.
(3pts) A student added sodium carbonate to a reaction tube and the color remained clear.
Propose
three specific reasons why the student saw no color change.
6.
(3pts) Increasing the temperature of a chemical reaction generally favors product formation.
Propose a hypothesis for why the 75°C reaction conditions did not produce the most activity.
2-2
