Lab Report_Bacterial Isolation_final

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NAME: Mason Patterson DATE: 12/26/23 Lab Report Bacterial Isolation This lab report is for you to reflect on what you completed and learned in this simulation, and to practice your written scientific communication skills. Include enough detail to clearly describe the lab experience to someone who understands the concepts but has not participated in the simulation. Use correct scientific terminology whenever applicable. Sections 1. Describe the overall objective and make a hypothesis 2. Introduce relevant background knowledge on this topic 3. Summarize the steps taken in the simulation 4. Explain any obtained results 5. Discuss the conclusions and implications 1 Copyright Labster ApS 2021 All Rights Reserved

1. Describe the overall objective and make a hypothesis. What is the overall purpose of the experiments or activities? Make a hypothesis. Recall that the hypothesis is a claim that answers a question or makes a prediction about an event that has not yet occurred. Hint: The purpose is often stated in the welcome message of the simulation (as shown in the example image below). For your entry make sure to include the following: ● The objective of the lab is to isolate one bacterial colony from a mixed culture. ● I hypothesize the bacteria responsible for the infection is Salmonella, which can be tested by growing and isolating from the sample collected at the chicken farm. 2 Copyright Labster ApS 2021 All Rights Reserved

2. Introduce relevant background knowledge on this topic. What have you learned in class or researched on your own that would help prepare for this simulation? Hint: You can review the “THEORY” section in the simulation (as shown in the example image below) For your entry make sure to include the following: ● Salmonella, a gram-negative bacterium, is commonly found in the digestive systems of animals, which causes Salmonellosis in humans when contaminated meat is improperly cooked. ● Lab cleaning procedures are crucial in a biological laboratory. Using PPE, cleaning with alcohol, and sterilizing with heat are all precautionary steps to prevent contamination from the outside world and preventing infection to lab personnel. ● In this lab, Salmonella colonies were isolated via streaking. This method uses a growth medium such as agar to inoculate bacteria by swiping an inoculating loop across the dish. The “T” method was used to decrease concentration of bacteria on the medium, allowing isolated colonies to be identified as Salmonella. 3 Copyright Labster ApS 2021 All Rights Reserved

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3. Summarize the steps taken in the simulation. Explain all steps you completed, including the equipment and techniques you used. Hint: You can use the “MISSION” tab in the LabPad as inspiration (as shown in the example image below). For your entry make sure to include the following: ● Max’s symptoms were identified as a bacterial infection, and his blood was taken. ● Bacteria from his blood was grown in a Salmonella Shigella plate. ● Bacterial colonies were confirmed to be Salmonella due to the color of the colonies. ● Samples of feces collected from the chicken shed floor were taken from the facility where the chicken eaten by Max was raised. ● Using the Bunsen burner, the wire loop was sterilized and then cooled on the agar plate. The wire loop was then dipped into the sample and streaked on the Salmonella agar plate. The tool was then disinfected with the Bunsen burner. ● The agar was placed upside down in the incubator overnight. ● Using the “T” method, colonies of Salmonella were isolated. This method covers approximately half of the plate in a concentrated streaking, then the loop intercepts 1-2 times, dragging a smaller amount of the streaked bacteria onto the bottom right quadrant, and then this step is repeated from the bottom right quadrant onto the bottom left quadrant. ● An isolated Salmonella colony was collected with the loop and placed into a culture tube. 5 Copyright Labster ApS 2021 All Rights Reserved

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4. Describe any obtained results. Explain all obtained results. Were these results expected or unexpected? Hint: You can use the “MEDIA” tab in the Lab Pad to revisit relevant images from the simulation (as shown in the example image below). For your entry make sure to include the following: ● Both Salmonella and E. Coli were found in the initial streaking. I was surprised we did not investigate E. Coli as well as Salmonella, since it is a common cause of foodborne illness. However, that was not the goal of the lab. The goal was to isolate a colony, which was done successfully by applying the T method of streaking in order to reduce the density of colonies, which allowed isolation and collection of the identified Salmonella black dot. 7 Copyright Labster ApS 2021 All Rights Reserved

5. Discuss the conclusions and implications. How do your results relate back to the original purpose and your hypothesis? Were there any systematic sources of error that could have affected the results? What did you learn? For your entry make sure to include the following: ● This simulation demonstrates my purpose and hypothesis by using a sample with many bacteria and isolating it using the T streak method. The hypothesis was proven because the isolated colony was Salmonella. ● In my simulation, I did not experience any errors. Possible avenues for making mistakes in this exercise would be with sterilization, such as failing to use the Bunsen burner to sterilize the probe. In a real-world lab setting, it would likely be best to take multiple samples of feces and performing this test on several agar plates for each sample. In the real world it would also be easier to make mistakes while streaking, such as not streaking sufficiently in the top-half of the T agar plate. ● From this simulation, I have learned the importance of sterilization and PPE. Salmonella is something you do not want to be infected with, so taking adequate precautions is especially important here. I learned how to isolate colonies of bacteria via streaking, along with how to correctly incubate an agar plate inoculated with bacteria. 9 Copyright Labster ApS 2021 All Rights Reserved

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