Lab 3 Assignment - Diffusion & Osmosis Fall2023

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Feb 20, 2024

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Names: Section # & Bench #: COMPLETE AS A PAIR! Lab 3 - Diffusion & Osmosis ( 15 points ) Due: By next week’s lab ● Complete this sheet, download as Word doc, submit on Canvas Please complete the following questions using your data from today’s lab. 1. Enter your data from Tables 1, 2 & 3 - answer the questions below: ( 3 pts ) Table 1: Speed of Diffusion through liquid Medium Elapsed Time (min) Radius (mm) Speed of Diffusion (mm/min) Liquid Table 2: Speed of Diffusion through Air Medium Distance to lab bench (mm) Elapsed Time (min) Speed of Diffusion (mm/min) Air Table 3: Speed of Diffusion through a Semisolid Medium Start Time End Time Radius (mm) Elapsed Time (min) Speed of Diffusion (mm/min) Semisolid 1a) In which experiment (medium) was diffusion the fastest ? 1b) What factors or variables account for the differences in speed? Explain by comparing any variables that may account for the differences in speed of diffusion between liquid, air, and a 1
Names: Section # & Bench #: COMPLETE AS A PAIR! semisolid. Be specific and use terms that are specific to what the molecules themselves are doing in this experiment. 2. Enter your data from Tables 4, 5, and 6; answer the questions below: ( 7 pts ) Table 4: Initial and Final Colors of solutions in Bag and Beaker Solution Original Contents Original Color Final Color Bag Beaker Table 5: Iodine I 2 KI Test for Presence of Starch Solution Source Original Contents Original Color Color After Iodine Test +/- Test for Starch Bag Beaker Control Table 6: Benedicts Test for Presence of Glucose Solution Source Original Contents Original Color Color After Benedicts Test +/- Test for Sugar Bag Beaker Control 2
Names: Section # & Bench #: COMPLETE AS A PAIR! 2a. Did starch, glucose, and the I 2 KI molecules diffuse through the dialysis tubing? How do you know? Explain how you know by interpreting your results from Tables 4, 5, and 6. Do not simply restate your results. 2b. Is the dialysis tubing semipermeable? How do you know? Explain based on your results for I 2 KI, starch and glucose. 2c. Estimate and compare the relative sizes of I 2 KI, glucose and starch molecules. Explain how you reached this estimate based the results of the diffusion experiment with the dialysis tubing. 2d. What colors would you expect if the experiment started with glucose and I 2 KI inside the bag and starch in the beaker? Explain. 3. Enter your data from Table 7; answer the questions below. You may include your sketches of cells ( 3 pts ): Table 7: Appearance of Elodea Cells in Test Solutions Solution Appearance/ Condition of Cells A B C 3a. Explain how the appearance of the Elodea cells allowed you to determine the tonicity of the unknown solutions. Include how solute concentration regulates the net direction of osmosis in your explanation. Refer to the textbook if you need help with language! 3b. Compare the responses of plant cells placed in hypertonic and hypotonic solutions. Explain the differences by referring to the structure of plant cells. 3
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Names: Section # & Bench #: COMPLETE AS A PAIR! 4. Enter your observations from Table 8 and answer the question below ( 2 pts ) Table 8: Tonicity & Turgor Pressure in Celery. Beaker Description of celery (flaccid/plasmolyzed) or turgid) Tonicity of Solution Explanation of net water movement direction A B 4a. Unlike animals, plants never absorb enough water that their cells burst, but they frequently lose enough water to wilt. Describe plant wilting in terms of turgor pressure, using your observations of celery. What can you conclude about the optimum tonicity for plants? (If you were a plant, which solution would you rather be in?!) 4

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