Homework Week 1 2

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Santa Ana College *

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315

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Biology

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Feb 20, 2024

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Name _________________________ Date______________ CLS 315 Molecular Diagnostics Week 1 Homework 15pts 1. Cellular Location and Activity of RNA Polymerase in Eukaryotes. List the types and subcellular location of each. (1.5pts) 2. Write the complimentary sequence to the following: 3’GGCATGCAG5’ (1.5pts) 3. A plasmid was digested with the enzyme NotI . On agarose gel electrophoresis you observe three bands 500bp 1000bp, and 1500bp. (4pts) a. How many NotI sites are present in this plasmid? b. What are the distances between each site? c. What is the size of the plasmid? d. Draw a picture of the plasmid with the NotI sites. 4. Calculate the nucleic acid concentration in μg/mL from the following information: (4pts) a. DNA: A reading at 260 nm = 0.5 b. RNA: A reading at 260 nm = 0.25 c. DNA: A reading at 260 nm from a 1:100 dilution = 0.12 d. RNA: A reading at 260 nm from a 1:200 dilution = 0.33 5. Calculate the yield for (a-d in question 4), if the volume of the solutions was 0.25ml. (4pt)
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