Homework Week 1-katecoffman-dr.pineda

d. Draw a picture of the plasmid with the NotI sites. 5’- - - - - - - - - - Notl - - - - - - - - - - 3’ ‘3 - - - - - - - - - - Notl - - - - - - - - - 5’  The distance between the Notl site and the end of the plasmid is 500bp on each side. 4. Calculate the nucleic acid concentration in μg/mL from the following information: (4pts) a. DNA: A reading at 260 nm = 0.5  Concentration of DNA = 0.5 x 1 x 50 = 25 ug / ml b. RNA: A reading at 260 nm = 0.25  Concentration of RNA = 0.25 X 1 X 40 = 10 ug / ml c. DNA: A reading at 260 nm from a 1:100 dilution = 0.12  Concentration of DNA (diluted) = 0.12 x 100 x 50 = 600 ug ml d. RNA: A reading at 260 nm from a 1:200 dilution = 0.33 Concentration of RNA (diluted) = 0.33 x 200 x 40 = 2640 ug / ml 5. Calculate the yield for (a-d in question 4), if the volume of the solutions was 0.25ml. (4pt) a. Yield of DNA ¿ 25 X 0.25 = 6.25 ug b. Yield of RNA ¿ 10 X 0.25 = 2.5 ug c. Yield of DNA (diluted) ¿ 600 x 0.25 = 150 ug d. Yield of RNA (diluted) ¿ 2640 x 0.25 = 660 ug