Ch 5 Mitosis in Onion Root Cells

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EUCs tasite,~ th Blue Tent Online Paine, Instructor Honors Biology - Chapter 5 4. Using the high-power lens, focus on the root’s tip. Find examples of cells in each stage of the cell cycle, including interphase and the stages of mitosis—prophase, metaphase, anaphase, and telophase. 5. Using a microscopic data sheet (attached), draw and label the different cells with the appropriate stage of mitosis (see attached sketch). 6. Select a random area of the slide to study using the high-power lens. 7. Identify the stage of each cell in the view and record your data in Table 1. TABLE 1: NUMBER OF CELLS IN EACH PHASE OF MITOSIS [ [ Total | Sample Interphase Metaphase Anaphase Telophase number | i | of cells . 26 | 16 | 0 | 0 in - i 2 114 | %2 | 1% | o 304 3 160 | %o ey | % ol 8. Move the slide around and repeat steps #6 and #7 two more times and record your information on Table 1 as Sample 2 and Sample 3. 9. Using the information from Table 1 and the calculations below, determine the percentage of cells in each phase of the cell cycle for each sample. Fill in Table 2. Sample calculation: # of cells in interphase — ! el x 100 =% of cells in interphase Total # of cells in each phase for sample 1 o TABLE2.STAGES OF THE CELL CYCLE B o ["Sample } Total lnterphnsz % \ Metaphase | % | Anaphase | % elophnse T % | | number of | | cells | ‘ | ©Hoh-McDougal Boogy - ol Suucture and Function T | |

Blue Tent Online Paine, Instructor Honors Biology - Chapter 5 Examples: Table 1: Number of Cells in Each Phase of Mitosis [ | Total Sample | Interphase Metaphase | Anaphase Telophase | number of cells. 248 i 3] s | e 2 | s 259 27 3 3 5 288 Table 2: Stages of the Cell Cycle Total | | | Sample | number Interphase % | Metaphase | % | Anaphase | % | Telophase | % oofeells | | B (N — | 1 257 248 | 9% | 1 0.4 3 1 5 2 | [ 2 259 252 |97 | 1 0.4 1 0.4 5 2 | [ 3 288 277 | 9 | 3 1 3 1 8 3 | ANALYZE AND CONCLUDE 1. Identify What patterns exist in your data? In which stage of the cell cycle are most of the cells you examined? How does this data support what you know about the cell cycle? Wt Al ot A Me atls were f ot Jor Pl pleplone, Tl oo Attanee wolbelh getiy phowt My il bininy " 0ok ppeducms, Myi gl eIl fumilin. . Ot McDougs Bolgy - Cell Stctur and Funcion

EUiC WaasheHer Blue Tent Online Paine, Instructor Honors Biology - Chapter 5 2. Caleulate Find the average percentage of cells in each stage of the cell cycle among the three samples. o Assume that a cell takes 24 hours to complete one cell cycle. o Caleulate how much time is spent in each stage of the cell cycle (Hint: Multiply the percentage of cells in cach stage, as a decimal, by 24 hours.) o Example: Interphase 96% (from Table 2) = 0.96 * 24 hours = 23.04 = ~23 hours. « Show your work! D24 Wbz 22 S4lpnn ;03329 bsun= P 7o, 0.57 2 gz (2.49] e Q256 1752 + 12,400 % = 1,52 bouen 12,52 [24 = 033 (33% tnlaferplae) 00S+ DI0+0.,00 = O\S 32 0.05( 5% MeSapdare) 0.00+ 0154023 = OU(1%kn haplose 0.00 +0.00 1026+ 0.08(g4 & Teleylsd 3. Predict: The cells in the root of an onion are actively dividing. How might the numbers you count here be different than if you had examined cells from a different part of the i 29 wrold preled Ay e guren (o alle gty oo Moy Moo iliglor o o pacs ard inoMtes yoort @m(a%wzmdpm% e han e dypdss, oo Mo covde, f Ho grio CHol McDoug By - ok e s urcion

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Ellvs luste, ~Hen Blue Tent Online Paine, Instructor Honors Biology - Chapter 5 4. Apply A chemical company is testing a new product that it believes will increase the growth rate of food plants. Suppose you are able to view the slides of onion root tips that have been treated with the product. If the product is successful, how might the slides look different from the slides you viewed in this lab? Ve Ledo ynitl prtrbly onbiss vupe cotls Mat 0t doer i Nofoghase Avaplor gt Telyloce Ly pon o \Megplios [ dhorl Ghoss tasr St Ve W pegre o0 2l f?/lam coll Apinni'm wliich il il Mo bin oyrr Apde. O Mcgn gy - ot rucursand Funcrion

Instructor: Irene Paine Student Name: _(=[11 | fus, Lo, Hn Date: _((//0/20 Lab Experiment Name and #: Mo o i it Cles Name of sample: Al win) rrt :£ »___ Collected from: e o Stain: — Mount: | S Lighting: v/ ( Observations: Magnification: ﬂ Magnification: Magnification:

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